Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity

Terminal deoxynucleotidyltransferase (TdT), which template-independently synthesizes DNA during V(D)J recombination in lymphoid cells, is ubiquitylated by a BPOZ-2/Cul3 complex, as the ubiquitin ligase, and then degraded by the 26 S proteasome. We show here that TdT is ubiquitylated by the Cul3-base...

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Autores principales: Maezawa, So, Fukushima, Rie, Matsushita, Toyofumi, Kato, Tomoyoshi, Takagaki, Yoshiki, Nishiyama, Yoshihiro, Ando, Sachiko, Matsumoto, Takuro, Kouda, Kousuke, Hayano, Takahide, Suzuki, Masahiro, Koiwai, Kotaro, Koiwai, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394778/
https://www.ncbi.nlm.nih.gov/pubmed/22808041
http://dx.doi.org/10.1371/journal.pone.0039511
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author Maezawa, So
Fukushima, Rie
Matsushita, Toyofumi
Kato, Tomoyoshi
Takagaki, Yoshiki
Nishiyama, Yoshihiro
Ando, Sachiko
Matsumoto, Takuro
Kouda, Kousuke
Hayano, Takahide
Suzuki, Masahiro
Koiwai, Kotaro
Koiwai, Osamu
author_facet Maezawa, So
Fukushima, Rie
Matsushita, Toyofumi
Kato, Tomoyoshi
Takagaki, Yoshiki
Nishiyama, Yoshihiro
Ando, Sachiko
Matsumoto, Takuro
Kouda, Kousuke
Hayano, Takahide
Suzuki, Masahiro
Koiwai, Kotaro
Koiwai, Osamu
author_sort Maezawa, So
collection PubMed
description Terminal deoxynucleotidyltransferase (TdT), which template-independently synthesizes DNA during V(D)J recombination in lymphoid cells, is ubiquitylated by a BPOZ-2/Cul3 complex, as the ubiquitin ligase, and then degraded by the 26 S proteasome. We show here that TdT is ubiquitylated by the Cul3-based ubiquitylation system in vitro. Because TdT could also be ubiquitylated in the absence of Cul/BPOZ-2, we determined that it could also be directly ubiquitylated by the E2 proteins UbcH5a/b/c and UbcH6, E3-independently. Furthermore, the ubiquitylated TdT inhibited its nucleotidyltransferase activity.
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spelling pubmed-33947782012-07-17 Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity Maezawa, So Fukushima, Rie Matsushita, Toyofumi Kato, Tomoyoshi Takagaki, Yoshiki Nishiyama, Yoshihiro Ando, Sachiko Matsumoto, Takuro Kouda, Kousuke Hayano, Takahide Suzuki, Masahiro Koiwai, Kotaro Koiwai, Osamu PLoS One Research Article Terminal deoxynucleotidyltransferase (TdT), which template-independently synthesizes DNA during V(D)J recombination in lymphoid cells, is ubiquitylated by a BPOZ-2/Cul3 complex, as the ubiquitin ligase, and then degraded by the 26 S proteasome. We show here that TdT is ubiquitylated by the Cul3-based ubiquitylation system in vitro. Because TdT could also be ubiquitylated in the absence of Cul/BPOZ-2, we determined that it could also be directly ubiquitylated by the E2 proteins UbcH5a/b/c and UbcH6, E3-independently. Furthermore, the ubiquitylated TdT inhibited its nucleotidyltransferase activity. Public Library of Science 2012-07-11 /pmc/articles/PMC3394778/ /pubmed/22808041 http://dx.doi.org/10.1371/journal.pone.0039511 Text en Maezawa et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Maezawa, So
Fukushima, Rie
Matsushita, Toyofumi
Kato, Tomoyoshi
Takagaki, Yoshiki
Nishiyama, Yoshihiro
Ando, Sachiko
Matsumoto, Takuro
Kouda, Kousuke
Hayano, Takahide
Suzuki, Masahiro
Koiwai, Kotaro
Koiwai, Osamu
Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity
title Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity
title_full Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity
title_fullStr Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity
title_full_unstemmed Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity
title_short Ubiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity
title_sort ubiquitylation of terminal deoxynucleotidyltransferase inhibits its activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394778/
https://www.ncbi.nlm.nih.gov/pubmed/22808041
http://dx.doi.org/10.1371/journal.pone.0039511
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