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Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway

In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-r...

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Autores principales: Belén Sanz, A., García, Raúl, Rodríguez-Peña, Jose Manuel, Díez-Muñiz, Sonia, Nombela, César, Peterson, Craig L., Arroyo, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395667/
https://www.ncbi.nlm.nih.gov/pubmed/22621902
http://dx.doi.org/10.1091/mbc.E12-04-0278
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author Belén Sanz, A.
García, Raúl
Rodríguez-Peña, Jose Manuel
Díez-Muñiz, Sonia
Nombela, César
Peterson, Craig L.
Arroyo, Javier
author_facet Belén Sanz, A.
García, Raúl
Rodríguez-Peña, Jose Manuel
Díez-Muñiz, Sonia
Nombela, César
Peterson, Craig L.
Arroyo, Javier
author_sort Belén Sanz, A.
collection PubMed
description In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress–responsive genes and hypersensitivity to cell wall–interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress.
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spelling pubmed-33956672012-09-30 Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway Belén Sanz, A. García, Raúl Rodríguez-Peña, Jose Manuel Díez-Muñiz, Sonia Nombela, César Peterson, Craig L. Arroyo, Javier Mol Biol Cell Articles In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress–responsive genes and hypersensitivity to cell wall–interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress. The American Society for Cell Biology 2012-07-15 /pmc/articles/PMC3395667/ /pubmed/22621902 http://dx.doi.org/10.1091/mbc.E12-04-0278 Text en © 2012 Sanz et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Belén Sanz, A.
García, Raúl
Rodríguez-Peña, Jose Manuel
Díez-Muñiz, Sonia
Nombela, César
Peterson, Craig L.
Arroyo, Javier
Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway
title Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway
title_full Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway
title_fullStr Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway
title_full_unstemmed Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway
title_short Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway
title_sort chromatin remodeling by the swi/snf complex is essential for transcription mediated by the yeast cell wall integrity mapk pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395667/
https://www.ncbi.nlm.nih.gov/pubmed/22621902
http://dx.doi.org/10.1091/mbc.E12-04-0278
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