Cargando…

Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores

A major barrier to complex experimental design in mouse genetics is the allele problem: combining three or more alleles is time-consuming and inefficient. Here, we solve this problem for transgenic animals with a simple modification of existing BAC transgenesis protocols, and generate triple-colored...

Descripción completa

Detalles Bibliográficos
Autores principales: Dougherty, Joseph D., Zhang, Juliet, Feng, Huifen, Gong, Shiaoching, Heintz, Nathaniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395707/
https://www.ncbi.nlm.nih.gov/pubmed/22808177
http://dx.doi.org/10.1371/journal.pone.0040511
_version_ 1782238022857654272
author Dougherty, Joseph D.
Zhang, Juliet
Feng, Huifen
Gong, Shiaoching
Heintz, Nathaniel
author_facet Dougherty, Joseph D.
Zhang, Juliet
Feng, Huifen
Gong, Shiaoching
Heintz, Nathaniel
author_sort Dougherty, Joseph D.
collection PubMed
description A major barrier to complex experimental design in mouse genetics is the allele problem: combining three or more alleles is time-consuming and inefficient. Here, we solve this problem for transgenic animals with a simple modification of existing BAC transgenesis protocols, and generate triple-colored ‘prism’ mice in which the major cell types of the brain: neurons, astrocytes, and oligodendrocytes, are each labeled with a distinct fluorophore. All three fluorophores are expressed from the same locus, yet each fluorophore is expressed in an independent temporal and spatial pattern. All three transgenes are generally co-inherited across multiple generations with stable genomic copy number and expression patterns. This generic solution should permit more sophisticated experimental manipulations to assess functional interactions amongst populations of cell types in vivo in a more rapid and efficient manner.
format Online
Article
Text
id pubmed-3395707
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-33957072012-07-17 Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores Dougherty, Joseph D. Zhang, Juliet Feng, Huifen Gong, Shiaoching Heintz, Nathaniel PLoS One Research Article A major barrier to complex experimental design in mouse genetics is the allele problem: combining three or more alleles is time-consuming and inefficient. Here, we solve this problem for transgenic animals with a simple modification of existing BAC transgenesis protocols, and generate triple-colored ‘prism’ mice in which the major cell types of the brain: neurons, astrocytes, and oligodendrocytes, are each labeled with a distinct fluorophore. All three fluorophores are expressed from the same locus, yet each fluorophore is expressed in an independent temporal and spatial pattern. All three transgenes are generally co-inherited across multiple generations with stable genomic copy number and expression patterns. This generic solution should permit more sophisticated experimental manipulations to assess functional interactions amongst populations of cell types in vivo in a more rapid and efficient manner. Public Library of Science 2012-07-12 /pmc/articles/PMC3395707/ /pubmed/22808177 http://dx.doi.org/10.1371/journal.pone.0040511 Text en Dougherty et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dougherty, Joseph D.
Zhang, Juliet
Feng, Huifen
Gong, Shiaoching
Heintz, Nathaniel
Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
title Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
title_full Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
title_fullStr Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
title_full_unstemmed Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
title_short Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
title_sort mouse transgenesis in a single locus with independent regulation for multiple fluorophores
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395707/
https://www.ncbi.nlm.nih.gov/pubmed/22808177
http://dx.doi.org/10.1371/journal.pone.0040511
work_keys_str_mv AT doughertyjosephd mousetransgenesisinasinglelocuswithindependentregulationformultiplefluorophores
AT zhangjuliet mousetransgenesisinasinglelocuswithindependentregulationformultiplefluorophores
AT fenghuifen mousetransgenesisinasinglelocuswithindependentregulationformultiplefluorophores
AT gongshiaoching mousetransgenesisinasinglelocuswithindependentregulationformultiplefluorophores
AT heintznathaniel mousetransgenesisinasinglelocuswithindependentregulationformultiplefluorophores