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Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
A major barrier to complex experimental design in mouse genetics is the allele problem: combining three or more alleles is time-consuming and inefficient. Here, we solve this problem for transgenic animals with a simple modification of existing BAC transgenesis protocols, and generate triple-colored...
Autores principales: | Dougherty, Joseph D., Zhang, Juliet, Feng, Huifen, Gong, Shiaoching, Heintz, Nathaniel |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395707/ https://www.ncbi.nlm.nih.gov/pubmed/22808177 http://dx.doi.org/10.1371/journal.pone.0040511 |
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