Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability

BACKGROUND: Recent studies have shown that the biological actions and toxicity of the water-soluble compound, polyhydroxyfullerene (fullerenol), are related to the concentrations present at a particular site of action. This study investigated the effects of different concentrations of fullerenol on...

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Detalles Bibliográficos
Autores principales: Zha, Ying-ying, Yang, Bo, Tang, Ming-liang, Guo, Qiu-chen, Chen, Ju-tao, Wen, Long-ping, Wang, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396393/
https://www.ncbi.nlm.nih.gov/pubmed/22802681
http://dx.doi.org/10.2147/IJN.S30934
Descripción
Sumario:BACKGROUND: Recent studies have shown that the biological actions and toxicity of the water-soluble compound, polyhydroxyfullerene (fullerenol), are related to the concentrations present at a particular site of action. This study investigated the effects of different concentrations of fullerenol on cultured rat hippocampal neurons. METHODS AND RESULTS: Fullerenol at low concentrations significantly enhanced hippocampal neuron viability as tested by MTT assay and Hoechst 33342/propidium iodide double stain detection. At high concentrations, fullerenol induced apoptosis confirmed by Comet assay and assessment of caspase proteins. CONCLUSION: These findings suggest that fullerenol promotes cell death and protects against cell damage, depending on the concentration present. The concentration-dependent effects of fullerenol were mainly due to its influence on the reduction-oxidation pathway.

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