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Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella

During the course of infection, Salmonella has to face several potentially lethal environmental conditions, one such being acidic pH. The ability to sense and respond to the acidic pH is crucial for the survival and replication of Salmonella. The physiological role of one gene (STM1485) involved in...

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Autores principales: Allam, Uday Sankar, Krishna, M. Gopala, Sen, Minakshi, Thomas, Rony, Lahiri, Amit, Gnanadhas, Divya Prakash, Chakravortty, Dipshikha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396692/
https://www.ncbi.nlm.nih.gov/pubmed/22460643
http://dx.doi.org/10.4161/viru.19029
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author Allam, Uday Sankar
Krishna, M. Gopala
Sen, Minakshi
Thomas, Rony
Lahiri, Amit
Gnanadhas, Divya Prakash
Chakravortty, Dipshikha
author_facet Allam, Uday Sankar
Krishna, M. Gopala
Sen, Minakshi
Thomas, Rony
Lahiri, Amit
Gnanadhas, Divya Prakash
Chakravortty, Dipshikha
author_sort Allam, Uday Sankar
collection PubMed
description During the course of infection, Salmonella has to face several potentially lethal environmental conditions, one such being acidic pH. The ability to sense and respond to the acidic pH is crucial for the survival and replication of Salmonella. The physiological role of one gene (STM1485) involved in this response, which is upregulated inside the host cells (by 90- to 113-fold) is functionally characterized in Salmonella pathogenesis. In vitro, the ΔSTM1485 neither exhibited any growth defect at pH 4.5 nor any difference in the acid tolerance response. The ΔSTM1485 was compromised in its capacity to proliferate inside the host cells and complementation with STM1485 gene restored its virulence. We further demonstrate that the surface translocation of Salmonella pathogenicity island-2 (SPI-2) encoded translocon proteins, SseB and SseD were reduced in the ΔSTM1485. The increase in co-localization of this mutant with lysosomes was also observed. In addition, the ΔSTM1485 displayed significantly reduced competitive indices (CI) in spleen, liver and mesenteric lymph nodes in murine typhoid model when infected by intra-gastric route. Based on these results, we conclude that the acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella.
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spelling pubmed-33966922012-07-16 Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella Allam, Uday Sankar Krishna, M. Gopala Sen, Minakshi Thomas, Rony Lahiri, Amit Gnanadhas, Divya Prakash Chakravortty, Dipshikha Virulence Research Paper During the course of infection, Salmonella has to face several potentially lethal environmental conditions, one such being acidic pH. The ability to sense and respond to the acidic pH is crucial for the survival and replication of Salmonella. The physiological role of one gene (STM1485) involved in this response, which is upregulated inside the host cells (by 90- to 113-fold) is functionally characterized in Salmonella pathogenesis. In vitro, the ΔSTM1485 neither exhibited any growth defect at pH 4.5 nor any difference in the acid tolerance response. The ΔSTM1485 was compromised in its capacity to proliferate inside the host cells and complementation with STM1485 gene restored its virulence. We further demonstrate that the surface translocation of Salmonella pathogenicity island-2 (SPI-2) encoded translocon proteins, SseB and SseD were reduced in the ΔSTM1485. The increase in co-localization of this mutant with lysosomes was also observed. In addition, the ΔSTM1485 displayed significantly reduced competitive indices (CI) in spleen, liver and mesenteric lymph nodes in murine typhoid model when infected by intra-gastric route. Based on these results, we conclude that the acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella. Landes Bioscience 2012-03-01 /pmc/articles/PMC3396692/ /pubmed/22460643 http://dx.doi.org/10.4161/viru.19029 Text en Copyright © 2012 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Allam, Uday Sankar
Krishna, M. Gopala
Sen, Minakshi
Thomas, Rony
Lahiri, Amit
Gnanadhas, Divya Prakash
Chakravortty, Dipshikha
Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella
title Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella
title_full Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella
title_fullStr Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella
title_full_unstemmed Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella
title_short Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella
title_sort acidic ph induced stm1485 gene is essential for intracellular replication of salmonella
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396692/
https://www.ncbi.nlm.nih.gov/pubmed/22460643
http://dx.doi.org/10.4161/viru.19029
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