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Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella
During the course of infection, Salmonella has to face several potentially lethal environmental conditions, one such being acidic pH. The ability to sense and respond to the acidic pH is crucial for the survival and replication of Salmonella. The physiological role of one gene (STM1485) involved in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396692/ https://www.ncbi.nlm.nih.gov/pubmed/22460643 http://dx.doi.org/10.4161/viru.19029 |
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author | Allam, Uday Sankar Krishna, M. Gopala Sen, Minakshi Thomas, Rony Lahiri, Amit Gnanadhas, Divya Prakash Chakravortty, Dipshikha |
author_facet | Allam, Uday Sankar Krishna, M. Gopala Sen, Minakshi Thomas, Rony Lahiri, Amit Gnanadhas, Divya Prakash Chakravortty, Dipshikha |
author_sort | Allam, Uday Sankar |
collection | PubMed |
description | During the course of infection, Salmonella has to face several potentially lethal environmental conditions, one such being acidic pH. The ability to sense and respond to the acidic pH is crucial for the survival and replication of Salmonella. The physiological role of one gene (STM1485) involved in this response, which is upregulated inside the host cells (by 90- to 113-fold) is functionally characterized in Salmonella pathogenesis. In vitro, the ΔSTM1485 neither exhibited any growth defect at pH 4.5 nor any difference in the acid tolerance response. The ΔSTM1485 was compromised in its capacity to proliferate inside the host cells and complementation with STM1485 gene restored its virulence. We further demonstrate that the surface translocation of Salmonella pathogenicity island-2 (SPI-2) encoded translocon proteins, SseB and SseD were reduced in the ΔSTM1485. The increase in co-localization of this mutant with lysosomes was also observed. In addition, the ΔSTM1485 displayed significantly reduced competitive indices (CI) in spleen, liver and mesenteric lymph nodes in murine typhoid model when infected by intra-gastric route. Based on these results, we conclude that the acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella. |
format | Online Article Text |
id | pubmed-3396692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-33966922012-07-16 Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella Allam, Uday Sankar Krishna, M. Gopala Sen, Minakshi Thomas, Rony Lahiri, Amit Gnanadhas, Divya Prakash Chakravortty, Dipshikha Virulence Research Paper During the course of infection, Salmonella has to face several potentially lethal environmental conditions, one such being acidic pH. The ability to sense and respond to the acidic pH is crucial for the survival and replication of Salmonella. The physiological role of one gene (STM1485) involved in this response, which is upregulated inside the host cells (by 90- to 113-fold) is functionally characterized in Salmonella pathogenesis. In vitro, the ΔSTM1485 neither exhibited any growth defect at pH 4.5 nor any difference in the acid tolerance response. The ΔSTM1485 was compromised in its capacity to proliferate inside the host cells and complementation with STM1485 gene restored its virulence. We further demonstrate that the surface translocation of Salmonella pathogenicity island-2 (SPI-2) encoded translocon proteins, SseB and SseD were reduced in the ΔSTM1485. The increase in co-localization of this mutant with lysosomes was also observed. In addition, the ΔSTM1485 displayed significantly reduced competitive indices (CI) in spleen, liver and mesenteric lymph nodes in murine typhoid model when infected by intra-gastric route. Based on these results, we conclude that the acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella. Landes Bioscience 2012-03-01 /pmc/articles/PMC3396692/ /pubmed/22460643 http://dx.doi.org/10.4161/viru.19029 Text en Copyright © 2012 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Research Paper Allam, Uday Sankar Krishna, M. Gopala Sen, Minakshi Thomas, Rony Lahiri, Amit Gnanadhas, Divya Prakash Chakravortty, Dipshikha Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella |
title | Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella |
title_full | Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella |
title_fullStr | Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella |
title_full_unstemmed | Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella |
title_short | Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella |
title_sort | acidic ph induced stm1485 gene is essential for intracellular replication of salmonella |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396692/ https://www.ncbi.nlm.nih.gov/pubmed/22460643 http://dx.doi.org/10.4161/viru.19029 |
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