A Protocol for Measurement of Noncoding RNA in Human Serum

MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Cu...

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Detalles Bibliográficos
Autores principales: Taylor, Caroline J., Satoor, Sarang N., Ranjan, Amaresh K., Pereira e Cotta, Maria V., Joglekar, Mugdha V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397160/
https://www.ncbi.nlm.nih.gov/pubmed/22811698
http://dx.doi.org/10.1155/2012/168368
Descripción
Sumario:MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.

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