A Protocol for Measurement of Noncoding RNA in Human Serum

MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Cu...

Descripción completa

Detalles Bibliográficos
Autores principales: Taylor, Caroline J., Satoor, Sarang N., Ranjan, Amaresh K., Pereira e Cotta, Maria V., Joglekar, Mugdha V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397160/
https://www.ncbi.nlm.nih.gov/pubmed/22811698
http://dx.doi.org/10.1155/2012/168368
_version_ 1782238154966695936
author Taylor, Caroline J.
Satoor, Sarang N.
Ranjan, Amaresh K.
Pereira e Cotta, Maria V.
Joglekar, Mugdha V.
author_facet Taylor, Caroline J.
Satoor, Sarang N.
Ranjan, Amaresh K.
Pereira e Cotta, Maria V.
Joglekar, Mugdha V.
author_sort Taylor, Caroline J.
collection PubMed
description MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.
format Online
Article
Text
id pubmed-3397160
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-33971602012-07-18 A Protocol for Measurement of Noncoding RNA in Human Serum Taylor, Caroline J. Satoor, Sarang N. Ranjan, Amaresh K. Pereira e Cotta, Maria V. Joglekar, Mugdha V. Exp Diabetes Res Methodology Report MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels. Hindawi Publishing Corporation 2012 2012-07-01 /pmc/articles/PMC3397160/ /pubmed/22811698 http://dx.doi.org/10.1155/2012/168368 Text en Copyright © 2012 Caroline J. Taylor et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Report
Taylor, Caroline J.
Satoor, Sarang N.
Ranjan, Amaresh K.
Pereira e Cotta, Maria V.
Joglekar, Mugdha V.
A Protocol for Measurement of Noncoding RNA in Human Serum
title A Protocol for Measurement of Noncoding RNA in Human Serum
title_full A Protocol for Measurement of Noncoding RNA in Human Serum
title_fullStr A Protocol for Measurement of Noncoding RNA in Human Serum
title_full_unstemmed A Protocol for Measurement of Noncoding RNA in Human Serum
title_short A Protocol for Measurement of Noncoding RNA in Human Serum
title_sort protocol for measurement of noncoding rna in human serum
topic Methodology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397160/
https://www.ncbi.nlm.nih.gov/pubmed/22811698
http://dx.doi.org/10.1155/2012/168368
work_keys_str_mv AT taylorcarolinej aprotocolformeasurementofnoncodingrnainhumanserum
AT satoorsarangn aprotocolformeasurementofnoncodingrnainhumanserum
AT ranjanamareshk aprotocolformeasurementofnoncodingrnainhumanserum
AT pereiraecottamariav aprotocolformeasurementofnoncodingrnainhumanserum
AT joglekarmugdhav aprotocolformeasurementofnoncodingrnainhumanserum
AT taylorcarolinej protocolformeasurementofnoncodingrnainhumanserum
AT satoorsarangn protocolformeasurementofnoncodingrnainhumanserum
AT ranjanamareshk protocolformeasurementofnoncodingrnainhumanserum
AT pereiraecottamariav protocolformeasurementofnoncodingrnainhumanserum
AT joglekarmugdhav protocolformeasurementofnoncodingrnainhumanserum

Ejemplares similares