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Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges
Genomic mining revealed one major nonribosomal peptide synthetase (NRPS) phylogenetic cluster in 12 marine sponge species, one ascidian, an actinobacterial isolate and seawater. Phylogenetic analysis predicts its taxonomic affiliation to the actinomycetes and hydroxy-phenyl-glycine as a likely subst...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397433/ https://www.ncbi.nlm.nih.gov/pubmed/22822366 http://dx.doi.org/10.3390/md10061192 |
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author | Pimentel-Elardo, Sheila Marie Grozdanov, Lubomir Proksch, Sebastian Hentschel, Ute |
author_facet | Pimentel-Elardo, Sheila Marie Grozdanov, Lubomir Proksch, Sebastian Hentschel, Ute |
author_sort | Pimentel-Elardo, Sheila Marie |
collection | PubMed |
description | Genomic mining revealed one major nonribosomal peptide synthetase (NRPS) phylogenetic cluster in 12 marine sponge species, one ascidian, an actinobacterial isolate and seawater. Phylogenetic analysis predicts its taxonomic affiliation to the actinomycetes and hydroxy-phenyl-glycine as a likely substrate. Additionally, a phylogenetically distinct NRPS gene cluster was discovered in the microbial metagenome of the sponge Aplysina aerophoba, which shows highest similarities to NRPS genes that were previously assigned, by ways of single cell genomics, to a Chloroflexi sponge symbiont. Genomic mining studies such as the one presented here for NRPS genes, contribute to on-going efforts to characterize the genomic potential of sponge-associated microbiota for secondary metabolite biosynthesis. |
format | Online Article Text |
id | pubmed-3397433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-33974332012-07-20 Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges Pimentel-Elardo, Sheila Marie Grozdanov, Lubomir Proksch, Sebastian Hentschel, Ute Mar Drugs Article Genomic mining revealed one major nonribosomal peptide synthetase (NRPS) phylogenetic cluster in 12 marine sponge species, one ascidian, an actinobacterial isolate and seawater. Phylogenetic analysis predicts its taxonomic affiliation to the actinomycetes and hydroxy-phenyl-glycine as a likely substrate. Additionally, a phylogenetically distinct NRPS gene cluster was discovered in the microbial metagenome of the sponge Aplysina aerophoba, which shows highest similarities to NRPS genes that were previously assigned, by ways of single cell genomics, to a Chloroflexi sponge symbiont. Genomic mining studies such as the one presented here for NRPS genes, contribute to on-going efforts to characterize the genomic potential of sponge-associated microbiota for secondary metabolite biosynthesis. MDPI 2012-05-25 /pmc/articles/PMC3397433/ /pubmed/22822366 http://dx.doi.org/10.3390/md10061192 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Pimentel-Elardo, Sheila Marie Grozdanov, Lubomir Proksch, Sebastian Hentschel, Ute Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges |
title | Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges |
title_full | Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges |
title_fullStr | Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges |
title_full_unstemmed | Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges |
title_short | Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine Sponges |
title_sort | diversity of nonribosomal peptide synthetase genes in the microbial metagenomes of marine sponges |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397433/ https://www.ncbi.nlm.nih.gov/pubmed/22822366 http://dx.doi.org/10.3390/md10061192 |
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