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Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig

Adipose tissue is not only a storage organ involved in fuel metabolism, but also an endocrine organ involved in the regulation of insulin sensitivity, thermogenesis, immunity, and inflammation. There are anatomical, cellular, molecular and physiological differences among adipose tissues deposited in...

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Detalles Bibliográficos
Autores principales: Li, Mingzhou, Wang, Tao, Wu, Honglong, Zhang, Jie, Zhou, Chaowei, Jiang, Anan, Li, Ruiqiang, Li, Xuewei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397513/
https://www.ncbi.nlm.nih.gov/pubmed/22837681
http://dx.doi.org/10.3390/ijms13067098
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author Li, Mingzhou
Wang, Tao
Wu, Honglong
Zhang, Jie
Zhou, Chaowei
Jiang, Anan
Li, Ruiqiang
Li, Xuewei
author_facet Li, Mingzhou
Wang, Tao
Wu, Honglong
Zhang, Jie
Zhou, Chaowei
Jiang, Anan
Li, Ruiqiang
Li, Xuewei
author_sort Li, Mingzhou
collection PubMed
description Adipose tissue is not only a storage organ involved in fuel metabolism, but also an endocrine organ involved in the regulation of insulin sensitivity, thermogenesis, immunity, and inflammation. There are anatomical, cellular, molecular and physiological differences among adipose tissues deposited in different body sites. However, current understanding of the intrinsic differences between the sub-compartments of the subcutaneous adipose tissue remains rudimentary. Here, we analyzed the genome-wide DNA methylation differences between the porcine superficial and deep backfat tissues using methylated DNA immunoprecipitation combined with high-throughput sequencing. We show that the genes with differentially methylated regions in their promoter are mainly involved in the processes of “lipid metabolism” and “regulation of immune-related cytokines”. Compared with the deep backfat tissue, the promoters of genes related to the ‘positive regulation of cytokine production’ were significantly hypermethylated in the superficial backfat tissue, which reflects the intrinsic functional and metabolic differences between the sub-compartments of the subcutaneous adipose tissue. This study provides epigenetic evidence for functionally relevant methylation differences between different layers of porcine backfat tissues.
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spelling pubmed-33975132012-07-26 Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig Li, Mingzhou Wang, Tao Wu, Honglong Zhang, Jie Zhou, Chaowei Jiang, Anan Li, Ruiqiang Li, Xuewei Int J Mol Sci Article Adipose tissue is not only a storage organ involved in fuel metabolism, but also an endocrine organ involved in the regulation of insulin sensitivity, thermogenesis, immunity, and inflammation. There are anatomical, cellular, molecular and physiological differences among adipose tissues deposited in different body sites. However, current understanding of the intrinsic differences between the sub-compartments of the subcutaneous adipose tissue remains rudimentary. Here, we analyzed the genome-wide DNA methylation differences between the porcine superficial and deep backfat tissues using methylated DNA immunoprecipitation combined with high-throughput sequencing. We show that the genes with differentially methylated regions in their promoter are mainly involved in the processes of “lipid metabolism” and “regulation of immune-related cytokines”. Compared with the deep backfat tissue, the promoters of genes related to the ‘positive regulation of cytokine production’ were significantly hypermethylated in the superficial backfat tissue, which reflects the intrinsic functional and metabolic differences between the sub-compartments of the subcutaneous adipose tissue. This study provides epigenetic evidence for functionally relevant methylation differences between different layers of porcine backfat tissues. Molecular Diversity Preservation International (MDPI) 2012-06-08 /pmc/articles/PMC3397513/ /pubmed/22837681 http://dx.doi.org/10.3390/ijms13067098 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Li, Mingzhou
Wang, Tao
Wu, Honglong
Zhang, Jie
Zhou, Chaowei
Jiang, Anan
Li, Ruiqiang
Li, Xuewei
Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig
title Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig
title_full Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig
title_fullStr Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig
title_full_unstemmed Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig
title_short Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig
title_sort genome-wide dna methylation changes between the superficial and deep backfat tissues of the pig
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397513/
https://www.ncbi.nlm.nih.gov/pubmed/22837681
http://dx.doi.org/10.3390/ijms13067098
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