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Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig
Adipose tissue is not only a storage organ involved in fuel metabolism, but also an endocrine organ involved in the regulation of insulin sensitivity, thermogenesis, immunity, and inflammation. There are anatomical, cellular, molecular and physiological differences among adipose tissues deposited in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397513/ https://www.ncbi.nlm.nih.gov/pubmed/22837681 http://dx.doi.org/10.3390/ijms13067098 |
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author | Li, Mingzhou Wang, Tao Wu, Honglong Zhang, Jie Zhou, Chaowei Jiang, Anan Li, Ruiqiang Li, Xuewei |
author_facet | Li, Mingzhou Wang, Tao Wu, Honglong Zhang, Jie Zhou, Chaowei Jiang, Anan Li, Ruiqiang Li, Xuewei |
author_sort | Li, Mingzhou |
collection | PubMed |
description | Adipose tissue is not only a storage organ involved in fuel metabolism, but also an endocrine organ involved in the regulation of insulin sensitivity, thermogenesis, immunity, and inflammation. There are anatomical, cellular, molecular and physiological differences among adipose tissues deposited in different body sites. However, current understanding of the intrinsic differences between the sub-compartments of the subcutaneous adipose tissue remains rudimentary. Here, we analyzed the genome-wide DNA methylation differences between the porcine superficial and deep backfat tissues using methylated DNA immunoprecipitation combined with high-throughput sequencing. We show that the genes with differentially methylated regions in their promoter are mainly involved in the processes of “lipid metabolism” and “regulation of immune-related cytokines”. Compared with the deep backfat tissue, the promoters of genes related to the ‘positive regulation of cytokine production’ were significantly hypermethylated in the superficial backfat tissue, which reflects the intrinsic functional and metabolic differences between the sub-compartments of the subcutaneous adipose tissue. This study provides epigenetic evidence for functionally relevant methylation differences between different layers of porcine backfat tissues. |
format | Online Article Text |
id | pubmed-3397513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-33975132012-07-26 Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig Li, Mingzhou Wang, Tao Wu, Honglong Zhang, Jie Zhou, Chaowei Jiang, Anan Li, Ruiqiang Li, Xuewei Int J Mol Sci Article Adipose tissue is not only a storage organ involved in fuel metabolism, but also an endocrine organ involved in the regulation of insulin sensitivity, thermogenesis, immunity, and inflammation. There are anatomical, cellular, molecular and physiological differences among adipose tissues deposited in different body sites. However, current understanding of the intrinsic differences between the sub-compartments of the subcutaneous adipose tissue remains rudimentary. Here, we analyzed the genome-wide DNA methylation differences between the porcine superficial and deep backfat tissues using methylated DNA immunoprecipitation combined with high-throughput sequencing. We show that the genes with differentially methylated regions in their promoter are mainly involved in the processes of “lipid metabolism” and “regulation of immune-related cytokines”. Compared with the deep backfat tissue, the promoters of genes related to the ‘positive regulation of cytokine production’ were significantly hypermethylated in the superficial backfat tissue, which reflects the intrinsic functional and metabolic differences between the sub-compartments of the subcutaneous adipose tissue. This study provides epigenetic evidence for functionally relevant methylation differences between different layers of porcine backfat tissues. Molecular Diversity Preservation International (MDPI) 2012-06-08 /pmc/articles/PMC3397513/ /pubmed/22837681 http://dx.doi.org/10.3390/ijms13067098 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Li, Mingzhou Wang, Tao Wu, Honglong Zhang, Jie Zhou, Chaowei Jiang, Anan Li, Ruiqiang Li, Xuewei Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig |
title | Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig |
title_full | Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig |
title_fullStr | Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig |
title_full_unstemmed | Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig |
title_short | Genome-Wide DNA Methylation Changes between the Superficial and Deep Backfat Tissues of the Pig |
title_sort | genome-wide dna methylation changes between the superficial and deep backfat tissues of the pig |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397513/ https://www.ncbi.nlm.nih.gov/pubmed/22837681 http://dx.doi.org/10.3390/ijms13067098 |
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