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Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer

Sox2 and Oct4 are transcription factors with the characteristics of regulating self-renewal and differentiation of embryonic stem cell. The aim of this study was to detect the expression of Sox2 and Oct4 and analyze their clinical significance in human non-small-cell lung cancer (NSCLC). Expression...

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Autores principales: Li, Xinxin, Wang, Jinguang, Xu, Zhiyun, Ahmad, Aftab, Li, Encheng, Wang, Yuan, Qin, Suli, Wang, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397552/
https://www.ncbi.nlm.nih.gov/pubmed/22837720
http://dx.doi.org/10.3390/ijms13067663
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author Li, Xinxin
Wang, Jinguang
Xu, Zhiyun
Ahmad, Aftab
Li, Encheng
Wang, Yuan
Qin, Suli
Wang, Qi
author_facet Li, Xinxin
Wang, Jinguang
Xu, Zhiyun
Ahmad, Aftab
Li, Encheng
Wang, Yuan
Qin, Suli
Wang, Qi
author_sort Li, Xinxin
collection PubMed
description Sox2 and Oct4 are transcription factors with the characteristics of regulating self-renewal and differentiation of embryonic stem cell. The aim of this study was to detect the expression of Sox2 and Oct4 and analyze their clinical significance in human non-small-cell lung cancer (NSCLC). Expression of Sox2 and Oct4 were assayed in cancer tissues and their corresponding paracancerous tissues from 44 patients with NSCLC and 21 patients with benign tumors using immunohistochemistry, Western blot, reverse transcription polymerase chain reaction (RT-PCR). The correlation between the expression of Sox2 and Oct4 and tumor type, grade and prognosis and the utility of the two genes in discriminating between benign and malignant tumors were analyzed as well. The results showed that Sox2 and Oct4 positive staining was only seen in the nuclei of cancer cells but not in either the precancerous tissues or benign tumor tissues by immunohistochemistry (p < 0.01). Furthermore, in the lung cancer tissue, the positive rate for Sox2 and Oct4 was 70.5% and 54.5%, respectively. Meanwhile, clinicopathological correlations showed that the Oct4 expression level was significantly associated with poorer differentiation and higher TNM stage of the cancer (p < 0.05). Western blot and RT-PCR analysis showed similar results to immunohistochemistry. Follow-up analysis revealed that expression of Oct4 was significantly associated with poor prognosis of lung cancer. The conclusion is that Sox2 and Oct4 may act as the promising unit markers in directing NSCLC diagnosis and therapy. Also, Oct4 can be regarded as a novel predictor of poor prognosis for NSCLC patients undergoing resection.
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spelling pubmed-33975522012-07-26 Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer Li, Xinxin Wang, Jinguang Xu, Zhiyun Ahmad, Aftab Li, Encheng Wang, Yuan Qin, Suli Wang, Qi Int J Mol Sci Article Sox2 and Oct4 are transcription factors with the characteristics of regulating self-renewal and differentiation of embryonic stem cell. The aim of this study was to detect the expression of Sox2 and Oct4 and analyze their clinical significance in human non-small-cell lung cancer (NSCLC). Expression of Sox2 and Oct4 were assayed in cancer tissues and their corresponding paracancerous tissues from 44 patients with NSCLC and 21 patients with benign tumors using immunohistochemistry, Western blot, reverse transcription polymerase chain reaction (RT-PCR). The correlation between the expression of Sox2 and Oct4 and tumor type, grade and prognosis and the utility of the two genes in discriminating between benign and malignant tumors were analyzed as well. The results showed that Sox2 and Oct4 positive staining was only seen in the nuclei of cancer cells but not in either the precancerous tissues or benign tumor tissues by immunohistochemistry (p < 0.01). Furthermore, in the lung cancer tissue, the positive rate for Sox2 and Oct4 was 70.5% and 54.5%, respectively. Meanwhile, clinicopathological correlations showed that the Oct4 expression level was significantly associated with poorer differentiation and higher TNM stage of the cancer (p < 0.05). Western blot and RT-PCR analysis showed similar results to immunohistochemistry. Follow-up analysis revealed that expression of Oct4 was significantly associated with poor prognosis of lung cancer. The conclusion is that Sox2 and Oct4 may act as the promising unit markers in directing NSCLC diagnosis and therapy. Also, Oct4 can be regarded as a novel predictor of poor prognosis for NSCLC patients undergoing resection. Molecular Diversity Preservation International (MDPI) 2012-06-21 /pmc/articles/PMC3397552/ /pubmed/22837720 http://dx.doi.org/10.3390/ijms13067663 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Li, Xinxin
Wang, Jinguang
Xu, Zhiyun
Ahmad, Aftab
Li, Encheng
Wang, Yuan
Qin, Suli
Wang, Qi
Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer
title Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer
title_full Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer
title_fullStr Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer
title_full_unstemmed Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer
title_short Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer
title_sort expression of sox2 and oct4 and their clinical significance in human non-small-cell lung cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397552/
https://www.ncbi.nlm.nih.gov/pubmed/22837720
http://dx.doi.org/10.3390/ijms13067663
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