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Watching DNA polymerase η make a phosphodiester bond
Native human DNA polymerase η, DNA and dATP were crystallized at pH 6.0 without Mg(2+). The polymerization reaction was initiated by exposing crystals to 1 mM Mg(2+) at pH 7.0, and stopped at various time points by freezing at 77 K for crystallographic analysis. The substrates and two Mg(2+) are ali...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397672/ https://www.ncbi.nlm.nih.gov/pubmed/22785315 http://dx.doi.org/10.1038/nature11181 |