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Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer

Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from the primary tumor into the bloodstream and, concordantly, the presence of these Circulating Tumor Cells (CTC) is associated with a poor prognosis. In this work, the molecular characterization of CTC from...

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Autores principales: Barbazán, Jorge, Alonso-Alconada, Lorena, Muinelo-Romay, Laura, Vieito, María, Abalo, Alicia, Alonso-Nocelo, Marta, Candamio, Sonia, Gallardo, Elena, Fernández, Beatriz, Abdulkader, Ihab, de los Ángeles Casares, María, Gómez-Tato, Antonio, López-López, Rafael, Abal, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397799/
https://www.ncbi.nlm.nih.gov/pubmed/22811761
http://dx.doi.org/10.1371/journal.pone.0040476
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author Barbazán, Jorge
Alonso-Alconada, Lorena
Muinelo-Romay, Laura
Vieito, María
Abalo, Alicia
Alonso-Nocelo, Marta
Candamio, Sonia
Gallardo, Elena
Fernández, Beatriz
Abdulkader, Ihab
de los Ángeles Casares, María
Gómez-Tato, Antonio
López-López, Rafael
Abal, Miguel
author_facet Barbazán, Jorge
Alonso-Alconada, Lorena
Muinelo-Romay, Laura
Vieito, María
Abalo, Alicia
Alonso-Nocelo, Marta
Candamio, Sonia
Gallardo, Elena
Fernández, Beatriz
Abdulkader, Ihab
de los Ángeles Casares, María
Gómez-Tato, Antonio
López-López, Rafael
Abal, Miguel
author_sort Barbazán, Jorge
collection PubMed
description Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from the primary tumor into the bloodstream and, concordantly, the presence of these Circulating Tumor Cells (CTC) is associated with a poor prognosis. In this work, the molecular characterization of CTC from mCRC patients was approached, with the aim of understanding their biology and improving their clinical utility in the management of colorectal cancer patients. For this, EpCAM-based immunoisolation of CTC was combined with whole transcriptome amplification and hybridization onto cDNA microarrays. Gene expression data from mCRC patients, once the background of unspecific immunoisolation from a group of controls had been subtracted, resulted in 410 genes that characterized the CTC population. Bioinformatics were used for the biological interpretation of the data, revealing that CTC are characterized by genes related to cell movement and adhesion, cell death and proliferation, and cell signalling and interaction. RTqPCR on an independent series of mCRC patients and controls was used for the validation of a number of genes related to the main cellular functions characterizing the CTC population. Comparison between primary carcinomas and lung and liver metastases further involved the CTC-genes in the promotion of metastasis. Moreover, the correlation of CTC-gene expression with clinical parameters demonstrated detection and prognosis significance. In conclusion, the molecular characterization of CTC from mCRC patients and the identification of diagnostic and prognostic biomarkers represent an innovative and promising approach in the clinical management of this type of patients.
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spelling pubmed-33977992012-07-18 Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer Barbazán, Jorge Alonso-Alconada, Lorena Muinelo-Romay, Laura Vieito, María Abalo, Alicia Alonso-Nocelo, Marta Candamio, Sonia Gallardo, Elena Fernández, Beatriz Abdulkader, Ihab de los Ángeles Casares, María Gómez-Tato, Antonio López-López, Rafael Abal, Miguel PLoS One Research Article Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from the primary tumor into the bloodstream and, concordantly, the presence of these Circulating Tumor Cells (CTC) is associated with a poor prognosis. In this work, the molecular characterization of CTC from mCRC patients was approached, with the aim of understanding their biology and improving their clinical utility in the management of colorectal cancer patients. For this, EpCAM-based immunoisolation of CTC was combined with whole transcriptome amplification and hybridization onto cDNA microarrays. Gene expression data from mCRC patients, once the background of unspecific immunoisolation from a group of controls had been subtracted, resulted in 410 genes that characterized the CTC population. Bioinformatics were used for the biological interpretation of the data, revealing that CTC are characterized by genes related to cell movement and adhesion, cell death and proliferation, and cell signalling and interaction. RTqPCR on an independent series of mCRC patients and controls was used for the validation of a number of genes related to the main cellular functions characterizing the CTC population. Comparison between primary carcinomas and lung and liver metastases further involved the CTC-genes in the promotion of metastasis. Moreover, the correlation of CTC-gene expression with clinical parameters demonstrated detection and prognosis significance. In conclusion, the molecular characterization of CTC from mCRC patients and the identification of diagnostic and prognostic biomarkers represent an innovative and promising approach in the clinical management of this type of patients. Public Library of Science 2012-07-10 /pmc/articles/PMC3397799/ /pubmed/22811761 http://dx.doi.org/10.1371/journal.pone.0040476 Text en Barbazán et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Barbazán, Jorge
Alonso-Alconada, Lorena
Muinelo-Romay, Laura
Vieito, María
Abalo, Alicia
Alonso-Nocelo, Marta
Candamio, Sonia
Gallardo, Elena
Fernández, Beatriz
Abdulkader, Ihab
de los Ángeles Casares, María
Gómez-Tato, Antonio
López-López, Rafael
Abal, Miguel
Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer
title Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer
title_full Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer
title_fullStr Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer
title_full_unstemmed Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer
title_short Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer
title_sort molecular characterization of circulating tumor cells in human metastatic colorectal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397799/
https://www.ncbi.nlm.nih.gov/pubmed/22811761
http://dx.doi.org/10.1371/journal.pone.0040476
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