Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer

We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε (585) (nm), 64,000 M(−1). cm(−1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring ac...

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Detalles Bibliográficos
Autores principales: Pettikiriarachchi, Anne, Gong, Lan, Perugini, Matthew A., Devenish, Rodney J., Prescott, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397996/
https://www.ncbi.nlm.nih.gov/pubmed/22815901
http://dx.doi.org/10.1371/journal.pone.0041028
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author Pettikiriarachchi, Anne
Gong, Lan
Perugini, Matthew A.
Devenish, Rodney J.
Prescott, Mark
author_facet Pettikiriarachchi, Anne
Gong, Lan
Perugini, Matthew A.
Devenish, Rodney J.
Prescott, Mark
author_sort Pettikiriarachchi, Anne
collection PubMed
description We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε (585) (nm), 64,000 M(−1). cm(−1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
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spelling pubmed-33979962012-07-19 Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer Pettikiriarachchi, Anne Gong, Lan Perugini, Matthew A. Devenish, Rodney J. Prescott, Mark PLoS One Research Article We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε (585) (nm), 64,000 M(−1). cm(−1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously. Public Library of Science 2012-07-16 /pmc/articles/PMC3397996/ /pubmed/22815901 http://dx.doi.org/10.1371/journal.pone.0041028 Text en Pettikiriarachchi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pettikiriarachchi, Anne
Gong, Lan
Perugini, Matthew A.
Devenish, Rodney J.
Prescott, Mark
Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer
title Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer
title_full Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer
title_fullStr Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer
title_full_unstemmed Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer
title_short Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer
title_sort ultramarine, a chromoprotein acceptor for förster resonance energy transfer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397996/
https://www.ncbi.nlm.nih.gov/pubmed/22815901
http://dx.doi.org/10.1371/journal.pone.0041028
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