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Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain
The formation of neural synapses according to the development and growth of neurite were usually studied with various markers. Of these markers, synaptophysin is a kind of synaptic protein located in the synaptic vesicle of neuron or neuroendocrine cell known to be distributed consistently in all ne...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association of Anatomists
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398181/ https://www.ncbi.nlm.nih.gov/pubmed/22822464 http://dx.doi.org/10.5115/acb.2012.45.2.97 |
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author | Pyeon, Hee-Jang Lee, Young-Il |
author_facet | Pyeon, Hee-Jang Lee, Young-Il |
author_sort | Pyeon, Hee-Jang |
collection | PubMed |
description | The formation of neural synapses according to the development and growth of neurite were usually studied with various markers. Of these markers, synaptophysin is a kind of synaptic protein located in the synaptic vesicle of neuron or neuroendocrine cell known to be distributed consistently in all neural synapses. The purpose of this study was to investigate differential expression levels and patterns of synaptic marker (synaptophysin) in the mouse hippocampal region according to the developmental stages of embryonic, neonatal, and adulthood respectively. In the embryonic and neonatal groups, synaptophysin immunofluorescence was almost defined to cornu ammonis subfields (CA1 and CA3) of hippocampus and subiculum proper in the hippocampal region. However in dentate gyrus, synaptophysin immunoreactivities were insignificant or absent in all developmental stages. In embryonic and neonatal hippocampus, the intensities of immunofluorescence were significantly different between molecular and oriens layers. Furthermore, those intensities were decreased considerably in both layers of neonatal group compared to embryonic. The results from this study will contribute to characterizing synaptogenic activities in the central nervous system through developmental stages. |
format | Online Article Text |
id | pubmed-3398181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Korean Association of Anatomists |
record_format | MEDLINE/PubMed |
spelling | pubmed-33981812012-07-20 Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain Pyeon, Hee-Jang Lee, Young-Il Anat Cell Biol Original Article The formation of neural synapses according to the development and growth of neurite were usually studied with various markers. Of these markers, synaptophysin is a kind of synaptic protein located in the synaptic vesicle of neuron or neuroendocrine cell known to be distributed consistently in all neural synapses. The purpose of this study was to investigate differential expression levels and patterns of synaptic marker (synaptophysin) in the mouse hippocampal region according to the developmental stages of embryonic, neonatal, and adulthood respectively. In the embryonic and neonatal groups, synaptophysin immunofluorescence was almost defined to cornu ammonis subfields (CA1 and CA3) of hippocampus and subiculum proper in the hippocampal region. However in dentate gyrus, synaptophysin immunoreactivities were insignificant or absent in all developmental stages. In embryonic and neonatal hippocampus, the intensities of immunofluorescence were significantly different between molecular and oriens layers. Furthermore, those intensities were decreased considerably in both layers of neonatal group compared to embryonic. The results from this study will contribute to characterizing synaptogenic activities in the central nervous system through developmental stages. Korean Association of Anatomists 2012-06 2012-06-30 /pmc/articles/PMC3398181/ /pubmed/22822464 http://dx.doi.org/10.5115/acb.2012.45.2.97 Text en Copyright © 2012. Anatomy & Cell Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Pyeon, Hee-Jang Lee, Young-Il Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain |
title | Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain |
title_full | Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain |
title_fullStr | Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain |
title_full_unstemmed | Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain |
title_short | Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain |
title_sort | differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398181/ https://www.ncbi.nlm.nih.gov/pubmed/22822464 http://dx.doi.org/10.5115/acb.2012.45.2.97 |
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