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Multimodal Protein Constructs for Herbivore Insect Control

Transgenic plants expressing combinations of microbial or plant pesticidal proteins represent a promising tool for the efficient, durable control of herbivorous insects. In this review we describe current strategies devised for the heterologous co-expression of pesticidal proteins in planta, some of...

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Autores principales: Sainsbury, Frank, Benchabane, Meriem, Goulet, Marie-Claire, Michaud, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398420/
https://www.ncbi.nlm.nih.gov/pubmed/22822457
http://dx.doi.org/10.3390/toxins4060455
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author Sainsbury, Frank
Benchabane, Meriem
Goulet, Marie-Claire
Michaud, Dominique
author_facet Sainsbury, Frank
Benchabane, Meriem
Goulet, Marie-Claire
Michaud, Dominique
author_sort Sainsbury, Frank
collection PubMed
description Transgenic plants expressing combinations of microbial or plant pesticidal proteins represent a promising tool for the efficient, durable control of herbivorous insects. In this review we describe current strategies devised for the heterologous co-expression of pesticidal proteins in planta, some of which have already shown usefulness in plant protection. Emphasis is placed on protein engineering strategies involving the insertion of single DNA constructs within the host plant genome. Multimodal fusion proteins integrating complementary pesticidal functions along a unique polypeptide are first considered, taking into account the structural constraints associated with protein or protein domain grafting to biologically active proteins. Strategies that allow for the co- or post-translational release of two or more pesticidal proteins are then considered, including polyprotein precursors releasing free proteins upon proteolytic cleavage, and multicistronic transcripts for the parallel translation of single protein-encoding mRNA sequences.
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spelling pubmed-33984202012-07-20 Multimodal Protein Constructs for Herbivore Insect Control Sainsbury, Frank Benchabane, Meriem Goulet, Marie-Claire Michaud, Dominique Toxins (Basel) Review Transgenic plants expressing combinations of microbial or plant pesticidal proteins represent a promising tool for the efficient, durable control of herbivorous insects. In this review we describe current strategies devised for the heterologous co-expression of pesticidal proteins in planta, some of which have already shown usefulness in plant protection. Emphasis is placed on protein engineering strategies involving the insertion of single DNA constructs within the host plant genome. Multimodal fusion proteins integrating complementary pesticidal functions along a unique polypeptide are first considered, taking into account the structural constraints associated with protein or protein domain grafting to biologically active proteins. Strategies that allow for the co- or post-translational release of two or more pesticidal proteins are then considered, including polyprotein precursors releasing free proteins upon proteolytic cleavage, and multicistronic transcripts for the parallel translation of single protein-encoding mRNA sequences. MDPI 2012-06-12 /pmc/articles/PMC3398420/ /pubmed/22822457 http://dx.doi.org/10.3390/toxins4060455 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Sainsbury, Frank
Benchabane, Meriem
Goulet, Marie-Claire
Michaud, Dominique
Multimodal Protein Constructs for Herbivore Insect Control
title Multimodal Protein Constructs for Herbivore Insect Control
title_full Multimodal Protein Constructs for Herbivore Insect Control
title_fullStr Multimodal Protein Constructs for Herbivore Insect Control
title_full_unstemmed Multimodal Protein Constructs for Herbivore Insect Control
title_short Multimodal Protein Constructs for Herbivore Insect Control
title_sort multimodal protein constructs for herbivore insect control
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398420/
https://www.ncbi.nlm.nih.gov/pubmed/22822457
http://dx.doi.org/10.3390/toxins4060455
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