Cargando…
Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice
Adiponectin is an adipose derived hormone that declines in obesity. We have previously shown that exogenous administration of adiponectin reduces allergic airways responses in mice. T-cadherin (T-cad; Cdh13) is a binding protein for the high molecular weight isoforms of adiponectin. To determine whe...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398886/ https://www.ncbi.nlm.nih.gov/pubmed/22815927 http://dx.doi.org/10.1371/journal.pone.0041088 |
_version_ | 1782238335092129792 |
---|---|
author | Williams, Alison S. Kasahara, David I. Verbout, Norah G. Fedulov, Alexey V. Zhu, Ming Si, Huiqing Wurmbrand, Allison P. Hug, Christopher Ranscht, Barbara Shore, Stephanie A. |
author_facet | Williams, Alison S. Kasahara, David I. Verbout, Norah G. Fedulov, Alexey V. Zhu, Ming Si, Huiqing Wurmbrand, Allison P. Hug, Christopher Ranscht, Barbara Shore, Stephanie A. |
author_sort | Williams, Alison S. |
collection | PubMed |
description | Adiponectin is an adipose derived hormone that declines in obesity. We have previously shown that exogenous administration of adiponectin reduces allergic airways responses in mice. T-cadherin (T-cad; Cdh13) is a binding protein for the high molecular weight isoforms of adiponectin. To determine whether the beneficial effects of adiponectin on allergic airways responses require T-cad, we sensitized wildtype (WT), T-cadherin deficient (T-cad(−/−)) and adiponectin and T-cad bideficient mice to ovalbumin (OVA) and challenged the mice with aerosolized OVA or PBS. Compared to WT, T-cad(−/−) mice were protected against OVA-induced airway hyperresponsiveness, increases in BAL inflammatory cells, and induction of IL-13, IL-17, and eotaxin expression. Histological analysis of the lungs of OVA-challenged T-cad(−/−) versus WT mice indicated reduced inflammation around the airways, and reduced mucous cell hyperplasia. Combined adiponectin and T-cad deficiency reversed the effects of T-cad deficiency alone, indicating that the observed effects of T-cad deficiency require adiponectin. Compared to WT, serum adiponectin was markedly increased in T-cad(−/−) mice, likely because adiponectin that is normally sequestered by endothelial T-cad remains free in the circulation. In conclusion, T-cad does not mediate the protective effects of adiponectin. Instead, mice lacking T-cad have reduced allergic airways disease, likely because elevated serum adiponectin levels act on other adiponectin signaling pathways. |
format | Online Article Text |
id | pubmed-3398886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33988862012-07-19 Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice Williams, Alison S. Kasahara, David I. Verbout, Norah G. Fedulov, Alexey V. Zhu, Ming Si, Huiqing Wurmbrand, Allison P. Hug, Christopher Ranscht, Barbara Shore, Stephanie A. PLoS One Research Article Adiponectin is an adipose derived hormone that declines in obesity. We have previously shown that exogenous administration of adiponectin reduces allergic airways responses in mice. T-cadherin (T-cad; Cdh13) is a binding protein for the high molecular weight isoforms of adiponectin. To determine whether the beneficial effects of adiponectin on allergic airways responses require T-cad, we sensitized wildtype (WT), T-cadherin deficient (T-cad(−/−)) and adiponectin and T-cad bideficient mice to ovalbumin (OVA) and challenged the mice with aerosolized OVA or PBS. Compared to WT, T-cad(−/−) mice were protected against OVA-induced airway hyperresponsiveness, increases in BAL inflammatory cells, and induction of IL-13, IL-17, and eotaxin expression. Histological analysis of the lungs of OVA-challenged T-cad(−/−) versus WT mice indicated reduced inflammation around the airways, and reduced mucous cell hyperplasia. Combined adiponectin and T-cad deficiency reversed the effects of T-cad deficiency alone, indicating that the observed effects of T-cad deficiency require adiponectin. Compared to WT, serum adiponectin was markedly increased in T-cad(−/−) mice, likely because adiponectin that is normally sequestered by endothelial T-cad remains free in the circulation. In conclusion, T-cad does not mediate the protective effects of adiponectin. Instead, mice lacking T-cad have reduced allergic airways disease, likely because elevated serum adiponectin levels act on other adiponectin signaling pathways. Public Library of Science 2012-07-17 /pmc/articles/PMC3398886/ /pubmed/22815927 http://dx.doi.org/10.1371/journal.pone.0041088 Text en Williams et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Williams, Alison S. Kasahara, David I. Verbout, Norah G. Fedulov, Alexey V. Zhu, Ming Si, Huiqing Wurmbrand, Allison P. Hug, Christopher Ranscht, Barbara Shore, Stephanie A. Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice |
title | Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice |
title_full | Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice |
title_fullStr | Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice |
title_full_unstemmed | Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice |
title_short | Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice |
title_sort | role of the adiponectin binding protein, t-cadherin (cdh13), in allergic airways responses in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398886/ https://www.ncbi.nlm.nih.gov/pubmed/22815927 http://dx.doi.org/10.1371/journal.pone.0041088 |
work_keys_str_mv | AT williamsalisons roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT kasaharadavidi roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT verboutnorahg roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT fedulovalexeyv roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT zhuming roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT sihuiqing roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT wurmbrandallisonp roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT hugchristopher roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT ranschtbarbara roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice AT shorestephaniea roleoftheadiponectinbindingproteintcadherincdh13inallergicairwaysresponsesinmice |