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Stem cell-related markers in primary breast cancers and associated metastatic lesions

It has been reported previously that: 1) normal breast epithelial cells that are CD24-/44+ express higher levels of stem/progenitor cell associated genes; 2) cancer cells that have undergone epithelial to mesenchymal transition display CD24-/44+ cell surface expression, a marker for breast cancer st...

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Autores principales: Guler, Gulnur, Balci, Serdar, Costinean, Stefan, Ussakli, Cigdem Himmetoglu, Irkkan, Cigdem, Suren, Dinc, Sari, Ebru, Altundag, Kadri, Ozisik, Yavuz, Jones, Susie, Bacher, Jason, Shapiro, Charles L., Huebner, Kay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400504/
https://www.ncbi.nlm.nih.gov/pubmed/22388757
http://dx.doi.org/10.1038/modpathol.2012.37
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author Guler, Gulnur
Balci, Serdar
Costinean, Stefan
Ussakli, Cigdem Himmetoglu
Irkkan, Cigdem
Suren, Dinc
Sari, Ebru
Altundag, Kadri
Ozisik, Yavuz
Jones, Susie
Bacher, Jason
Shapiro, Charles L.
Huebner, Kay
author_facet Guler, Gulnur
Balci, Serdar
Costinean, Stefan
Ussakli, Cigdem Himmetoglu
Irkkan, Cigdem
Suren, Dinc
Sari, Ebru
Altundag, Kadri
Ozisik, Yavuz
Jones, Susie
Bacher, Jason
Shapiro, Charles L.
Huebner, Kay
author_sort Guler, Gulnur
collection PubMed
description It has been reported previously that: 1) normal breast epithelial cells that are CD24-/44+ express higher levels of stem/progenitor cell associated genes; 2) cancer cells that have undergone epithelial to mesenchymal transition display CD24-/44+ cell surface expression, a marker for breast cancer stem cells; 3) loss of E-cadherin is a preliminary step in epithelial to mesenchymal transition; and 4) vimentin is a marker of mesenchymal phenotype. We hypothesized that stem cell subpopulations would be more frequent in metastatic than in primary tumors. Therefore we assessed by immunohistochemical analysis, tissue microarrays containing tissue from primary and associated metastatic breast cancers for expression of CD24, CD44, E-cadherin and vimentin to evaluate candidate cancer-initiating cell populations in breast cancer subtypes and metastatic lesions. The occurrence of CD24-/44+ and CD24+/44- cells did not differ in primary vs matched lymph node or distant and locoregional metastatic lesions; E-cadherin expression was decreased in primary vs lymph node metastases (P=0.018) but not decreased in distant and locoregional metastases relative to primary tumor, while vimentin, was more frequently expressed in lymph node and distant and locoregional metastases (P=0.013, P=0.004) than in matched primary cancers. Thus, the frequency of CD24-/44+ cells does not differ in metastases relative to the primary breast cancer but differs by tumor stage and subtype.
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spelling pubmed-34005042013-01-01 Stem cell-related markers in primary breast cancers and associated metastatic lesions Guler, Gulnur Balci, Serdar Costinean, Stefan Ussakli, Cigdem Himmetoglu Irkkan, Cigdem Suren, Dinc Sari, Ebru Altundag, Kadri Ozisik, Yavuz Jones, Susie Bacher, Jason Shapiro, Charles L. Huebner, Kay Mod Pathol Article It has been reported previously that: 1) normal breast epithelial cells that are CD24-/44+ express higher levels of stem/progenitor cell associated genes; 2) cancer cells that have undergone epithelial to mesenchymal transition display CD24-/44+ cell surface expression, a marker for breast cancer stem cells; 3) loss of E-cadherin is a preliminary step in epithelial to mesenchymal transition; and 4) vimentin is a marker of mesenchymal phenotype. We hypothesized that stem cell subpopulations would be more frequent in metastatic than in primary tumors. Therefore we assessed by immunohistochemical analysis, tissue microarrays containing tissue from primary and associated metastatic breast cancers for expression of CD24, CD44, E-cadherin and vimentin to evaluate candidate cancer-initiating cell populations in breast cancer subtypes and metastatic lesions. The occurrence of CD24-/44+ and CD24+/44- cells did not differ in primary vs matched lymph node or distant and locoregional metastatic lesions; E-cadherin expression was decreased in primary vs lymph node metastases (P=0.018) but not decreased in distant and locoregional metastases relative to primary tumor, while vimentin, was more frequently expressed in lymph node and distant and locoregional metastases (P=0.013, P=0.004) than in matched primary cancers. Thus, the frequency of CD24-/44+ cells does not differ in metastases relative to the primary breast cancer but differs by tumor stage and subtype. 2012-03-02 2012-07 /pmc/articles/PMC3400504/ /pubmed/22388757 http://dx.doi.org/10.1038/modpathol.2012.37 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Guler, Gulnur
Balci, Serdar
Costinean, Stefan
Ussakli, Cigdem Himmetoglu
Irkkan, Cigdem
Suren, Dinc
Sari, Ebru
Altundag, Kadri
Ozisik, Yavuz
Jones, Susie
Bacher, Jason
Shapiro, Charles L.
Huebner, Kay
Stem cell-related markers in primary breast cancers and associated metastatic lesions
title Stem cell-related markers in primary breast cancers and associated metastatic lesions
title_full Stem cell-related markers in primary breast cancers and associated metastatic lesions
title_fullStr Stem cell-related markers in primary breast cancers and associated metastatic lesions
title_full_unstemmed Stem cell-related markers in primary breast cancers and associated metastatic lesions
title_short Stem cell-related markers in primary breast cancers and associated metastatic lesions
title_sort stem cell-related markers in primary breast cancers and associated metastatic lesions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400504/
https://www.ncbi.nlm.nih.gov/pubmed/22388757
http://dx.doi.org/10.1038/modpathol.2012.37
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