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Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans

RNA interference (RNAi)–related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part r...

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Autores principales: Kamminga, Leonie M., van Wolfswinkel, Josien C., Luteijn, Maartje J., Kaaij, Lucas J. T., Bagijn, Marloes P., Sapetschnig, Alexandra, Miska, Eric A., Berezikov, Eugene, Ketting, René F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400576/
https://www.ncbi.nlm.nih.gov/pubmed/22829772
http://dx.doi.org/10.1371/journal.pgen.1002702
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author Kamminga, Leonie M.
van Wolfswinkel, Josien C.
Luteijn, Maartje J.
Kaaij, Lucas J. T.
Bagijn, Marloes P.
Sapetschnig, Alexandra
Miska, Eric A.
Berezikov, Eugene
Ketting, René F.
author_facet Kamminga, Leonie M.
van Wolfswinkel, Josien C.
Luteijn, Maartje J.
Kaaij, Lucas J. T.
Bagijn, Marloes P.
Sapetschnig, Alexandra
Miska, Eric A.
Berezikov, Eugene
Ketting, René F.
author_sort Kamminga, Leonie M.
collection PubMed
description RNA interference (RNAi)–related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part regulated by Hen1-mediated methylation of their 3′ ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA–methyl-transferase homolog, HENN-1, on the different RNAi pathways in this nematode. We reveal differential effects of HENN-1 on the two pathways that are known to employ methylated sRNA molecules: the 26G and 21U pathways. Surprisingly, in the germline, stability of 21U RNAs, the C. elegans piRNAs, is only mildly affected by loss of methylation; and introduction of artificial 21U target RNA does not further destabilize non-methylated 21U RNAs. In contrast, most 26G RNAs display reduced stability and respond to loss of HENN-1 by displaying increased 3′-uridylation frequencies. Within the 26G RNA class, we find that specifically ERGO-1–bound 26G RNAs are modified by HENN-1, while ALG-3/ALG-4–bound 26G RNAs are not. Global gene expression analysis of henn-1 mutants reveals mild effects, including down-regulation of many germline-expressed genes. Our data suggest that, apart from direct effects of reduced 26G RNA levels of henn-1 on gene expression, most effects on global gene expression are indirect. These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymes in these pathways.
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spelling pubmed-34005762012-07-24 Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans Kamminga, Leonie M. van Wolfswinkel, Josien C. Luteijn, Maartje J. Kaaij, Lucas J. T. Bagijn, Marloes P. Sapetschnig, Alexandra Miska, Eric A. Berezikov, Eugene Ketting, René F. PLoS Genet Research Article RNA interference (RNAi)–related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part regulated by Hen1-mediated methylation of their 3′ ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA–methyl-transferase homolog, HENN-1, on the different RNAi pathways in this nematode. We reveal differential effects of HENN-1 on the two pathways that are known to employ methylated sRNA molecules: the 26G and 21U pathways. Surprisingly, in the germline, stability of 21U RNAs, the C. elegans piRNAs, is only mildly affected by loss of methylation; and introduction of artificial 21U target RNA does not further destabilize non-methylated 21U RNAs. In contrast, most 26G RNAs display reduced stability and respond to loss of HENN-1 by displaying increased 3′-uridylation frequencies. Within the 26G RNA class, we find that specifically ERGO-1–bound 26G RNAs are modified by HENN-1, while ALG-3/ALG-4–bound 26G RNAs are not. Global gene expression analysis of henn-1 mutants reveals mild effects, including down-regulation of many germline-expressed genes. Our data suggest that, apart from direct effects of reduced 26G RNA levels of henn-1 on gene expression, most effects on global gene expression are indirect. These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymes in these pathways. Public Library of Science 2012-07-19 /pmc/articles/PMC3400576/ /pubmed/22829772 http://dx.doi.org/10.1371/journal.pgen.1002702 Text en Kamminga et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kamminga, Leonie M.
van Wolfswinkel, Josien C.
Luteijn, Maartje J.
Kaaij, Lucas J. T.
Bagijn, Marloes P.
Sapetschnig, Alexandra
Miska, Eric A.
Berezikov, Eugene
Ketting, René F.
Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
title Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
title_full Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
title_fullStr Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
title_full_unstemmed Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
title_short Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
title_sort differential impact of the hen1 homolog henn-1 on 21u and 26g rnas in the germline of caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400576/
https://www.ncbi.nlm.nih.gov/pubmed/22829772
http://dx.doi.org/10.1371/journal.pgen.1002702
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