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Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1)
Apical membrane antigen-1 (AMA1) is a micronemal protein of apicomplexan parasites that appears to be essential during the invasion of host cells. In this study, a full-length cDNA of AMA1 was identified from Eimeria tenella (Et) using expressed sequence tag and the rapid amplification of cDNA ends...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400601/ https://www.ncbi.nlm.nih.gov/pubmed/22829917 http://dx.doi.org/10.1371/journal.pone.0041115 |
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author | Jiang, Lianlian Lin, Jiaojiao Han, Hongyu Dong, Hui Zhao, Qiping Zhu, Shunhai Huang, Bing |
author_facet | Jiang, Lianlian Lin, Jiaojiao Han, Hongyu Dong, Hui Zhao, Qiping Zhu, Shunhai Huang, Bing |
author_sort | Jiang, Lianlian |
collection | PubMed |
description | Apical membrane antigen-1 (AMA1) is a micronemal protein of apicomplexan parasites that appears to be essential during the invasion of host cells. In this study, a full-length cDNA of AMA1 was identified from Eimeria tenella (Et) using expressed sequence tag and the rapid amplification of cDNA ends technique. EtAMA1 had an open reading frame of 1608 bp encoding a protein of 535 amino acids. Quantitative real-time PCR analysis revealed that EtAMA1 was expressed at higher levels in sporozoites than in the other developmental stages (unsporulated oocysts, sporulated oocysts and second-generation merozoites). The ectodomain sequence was expressed as recombinant EtAMA1 (rEtAMA1) and rabbit polyclonal antibodies raised against the rEtAMA1 recognized a 58-kDa native parasite protein by Western Blotting and had a potent inhibitory effect on parasite invasion, decreasing it by approximately 70%. Immunofluorescence analysis and immunohistochemistry analysis showed EtAMA1 might play an important role in sporozoite invasion and development. |
format | Online Article Text |
id | pubmed-3400601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34006012012-07-24 Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1) Jiang, Lianlian Lin, Jiaojiao Han, Hongyu Dong, Hui Zhao, Qiping Zhu, Shunhai Huang, Bing PLoS One Research Article Apical membrane antigen-1 (AMA1) is a micronemal protein of apicomplexan parasites that appears to be essential during the invasion of host cells. In this study, a full-length cDNA of AMA1 was identified from Eimeria tenella (Et) using expressed sequence tag and the rapid amplification of cDNA ends technique. EtAMA1 had an open reading frame of 1608 bp encoding a protein of 535 amino acids. Quantitative real-time PCR analysis revealed that EtAMA1 was expressed at higher levels in sporozoites than in the other developmental stages (unsporulated oocysts, sporulated oocysts and second-generation merozoites). The ectodomain sequence was expressed as recombinant EtAMA1 (rEtAMA1) and rabbit polyclonal antibodies raised against the rEtAMA1 recognized a 58-kDa native parasite protein by Western Blotting and had a potent inhibitory effect on parasite invasion, decreasing it by approximately 70%. Immunofluorescence analysis and immunohistochemistry analysis showed EtAMA1 might play an important role in sporozoite invasion and development. Public Library of Science 2012-07-19 /pmc/articles/PMC3400601/ /pubmed/22829917 http://dx.doi.org/10.1371/journal.pone.0041115 Text en Jiang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jiang, Lianlian Lin, Jiaojiao Han, Hongyu Dong, Hui Zhao, Qiping Zhu, Shunhai Huang, Bing Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1) |
title | Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1) |
title_full | Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1) |
title_fullStr | Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1) |
title_full_unstemmed | Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1) |
title_short | Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1) |
title_sort | identification and characterization of eimeria tenella apical membrane antigen-1 (ama1) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400601/ https://www.ncbi.nlm.nih.gov/pubmed/22829917 http://dx.doi.org/10.1371/journal.pone.0041115 |
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