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Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome

INTRODUCTION: Pathomechanism of HRS is still poorly understood. The aim of our study was: (1) to test whether different strains of rats could develop typical HRS, and (2) to estimate the influence of activation and inhibition of nitric oxide for development of renal failure in course of HRS. MATERIA...

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Autores principales: Saracyn, Marek, Patera, Janusz, Kocik, Janusz, Brytan, Marek, Zdanowski, Robert, Lubas, Arkadiusz, Kozłowski, Wojciech, Wańkowicz, Zofia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400905/
https://www.ncbi.nlm.nih.gov/pubmed/22852015
http://dx.doi.org/10.5114/aoms.2012.29281
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author Saracyn, Marek
Patera, Janusz
Kocik, Janusz
Brytan, Marek
Zdanowski, Robert
Lubas, Arkadiusz
Kozłowski, Wojciech
Wańkowicz, Zofia
author_facet Saracyn, Marek
Patera, Janusz
Kocik, Janusz
Brytan, Marek
Zdanowski, Robert
Lubas, Arkadiusz
Kozłowski, Wojciech
Wańkowicz, Zofia
author_sort Saracyn, Marek
collection PubMed
description INTRODUCTION: Pathomechanism of HRS is still poorly understood. The aim of our study was: (1) to test whether different strains of rats could develop typical HRS, and (2) to estimate the influence of activation and inhibition of nitric oxide for development of renal failure in course of HRS. MATERIAL AND METHODS: First, we used 16 of Wistar and 16 of Sprague-Dawley rats in galactosamine model of HRS. Next, we used 48 of SDR rats, which received saline, N-nitro-L-arginine or L-arginine before and after liver damage. Twenty four hours urine and blood samples were collected 48 h after saline or Ga1N injection. Biochemical parameters were determined in serum or urine and then creatinine clearance and osmolality clearance were calculated. Liver and kidney tissues were collected for histopathological examination. RESULTS: Liver failure developed in all tested groups with significant increase of bilirubin (p < 0.001), ALT (p < 0.001) and ammonia (p < 0.001). Nevertheless we did not achieve any evidence of renal failure in Wistar, but we found typical renal failure in Sprague-Dawley group with significant decrease in creatinine clearance (p < 0.0012) and increase in concentration of creatinine and urea (p < 0.001) and (p < 0.001) respectively. Inhibition of NOS prevented development of renal failure with significant improvement of GFR both before (p < 0.0017) and after (p < 0.003) Ga1N injection. Injection of L-arginine after Ga1N injection did not caused significant improvement of GFR. CONCLUSIONS: Our study showed, that genetic factors might be responsible for development of renal failure in course of HRS and nitric oxide play important role in acute model of this syndrome.
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spelling pubmed-34009052012-07-31 Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome Saracyn, Marek Patera, Janusz Kocik, Janusz Brytan, Marek Zdanowski, Robert Lubas, Arkadiusz Kozłowski, Wojciech Wańkowicz, Zofia Arch Med Sci Experimental Research INTRODUCTION: Pathomechanism of HRS is still poorly understood. The aim of our study was: (1) to test whether different strains of rats could develop typical HRS, and (2) to estimate the influence of activation and inhibition of nitric oxide for development of renal failure in course of HRS. MATERIAL AND METHODS: First, we used 16 of Wistar and 16 of Sprague-Dawley rats in galactosamine model of HRS. Next, we used 48 of SDR rats, which received saline, N-nitro-L-arginine or L-arginine before and after liver damage. Twenty four hours urine and blood samples were collected 48 h after saline or Ga1N injection. Biochemical parameters were determined in serum or urine and then creatinine clearance and osmolality clearance were calculated. Liver and kidney tissues were collected for histopathological examination. RESULTS: Liver failure developed in all tested groups with significant increase of bilirubin (p < 0.001), ALT (p < 0.001) and ammonia (p < 0.001). Nevertheless we did not achieve any evidence of renal failure in Wistar, but we found typical renal failure in Sprague-Dawley group with significant decrease in creatinine clearance (p < 0.0012) and increase in concentration of creatinine and urea (p < 0.001) and (p < 0.001) respectively. Inhibition of NOS prevented development of renal failure with significant improvement of GFR both before (p < 0.0017) and after (p < 0.003) Ga1N injection. Injection of L-arginine after Ga1N injection did not caused significant improvement of GFR. CONCLUSIONS: Our study showed, that genetic factors might be responsible for development of renal failure in course of HRS and nitric oxide play important role in acute model of this syndrome. Termedia Publishing House 2012-07-04 2012-07-04 /pmc/articles/PMC3400905/ /pubmed/22852015 http://dx.doi.org/10.5114/aoms.2012.29281 Text en Copyright © 2012 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Experimental Research
Saracyn, Marek
Patera, Janusz
Kocik, Janusz
Brytan, Marek
Zdanowski, Robert
Lubas, Arkadiusz
Kozłowski, Wojciech
Wańkowicz, Zofia
Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome
title Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome
title_full Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome
title_fullStr Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome
title_full_unstemmed Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome
title_short Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome
title_sort strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome
topic Experimental Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400905/
https://www.ncbi.nlm.nih.gov/pubmed/22852015
http://dx.doi.org/10.5114/aoms.2012.29281
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