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BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity

RPA is a single-stranded DNA binding protein that physically associates with the BLM complex. RPA stimulates BLM helicase activity as well as the double Holliday junction dissolution activity of the BLM-topoisomerase IIIα complex. We investigated the effect of RPA on the ssDNA decatenase activity of...

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Detalles Bibliográficos
Autores principales: Yang, Jay, Bachrati, Csanad Z., Hickson, Ian D., Brown, Grant W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401101/
https://www.ncbi.nlm.nih.gov/pubmed/22911760
http://dx.doi.org/10.1371/journal.pone.0041208
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author Yang, Jay
Bachrati, Csanad Z.
Hickson, Ian D.
Brown, Grant W.
author_facet Yang, Jay
Bachrati, Csanad Z.
Hickson, Ian D.
Brown, Grant W.
author_sort Yang, Jay
collection PubMed
description RPA is a single-stranded DNA binding protein that physically associates with the BLM complex. RPA stimulates BLM helicase activity as well as the double Holliday junction dissolution activity of the BLM-topoisomerase IIIα complex. We investigated the effect of RPA on the ssDNA decatenase activity of topoisomerase IIIα. We found that RPA and other ssDNA binding proteins inhibit decatenation by topoisomerase IIIα. Complex formation between BLM, TopoIIIα, and RMI1 ablates inhibition of decatenation by ssDNA binding proteins. Together, these data indicate that inhibition by RPA does not involve species-specific interactions between RPA and BLM-TopoIIIα-RMI1, which contrasts with RPA modulation of double Holliday junction dissolution. We propose that topoisomerase IIIα and RPA compete to bind to single-stranded regions of catenanes. Interactions with BLM and RMI1 enhance toposiomerase IIIα activity, promoting decatenation in the presence of RPA.
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spelling pubmed-34011012012-07-30 BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity Yang, Jay Bachrati, Csanad Z. Hickson, Ian D. Brown, Grant W. PLoS One Research Article RPA is a single-stranded DNA binding protein that physically associates with the BLM complex. RPA stimulates BLM helicase activity as well as the double Holliday junction dissolution activity of the BLM-topoisomerase IIIα complex. We investigated the effect of RPA on the ssDNA decatenase activity of topoisomerase IIIα. We found that RPA and other ssDNA binding proteins inhibit decatenation by topoisomerase IIIα. Complex formation between BLM, TopoIIIα, and RMI1 ablates inhibition of decatenation by ssDNA binding proteins. Together, these data indicate that inhibition by RPA does not involve species-specific interactions between RPA and BLM-TopoIIIα-RMI1, which contrasts with RPA modulation of double Holliday junction dissolution. We propose that topoisomerase IIIα and RPA compete to bind to single-stranded regions of catenanes. Interactions with BLM and RMI1 enhance toposiomerase IIIα activity, promoting decatenation in the presence of RPA. Public Library of Science 2012-07-20 /pmc/articles/PMC3401101/ /pubmed/22911760 http://dx.doi.org/10.1371/journal.pone.0041208 Text en Yang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yang, Jay
Bachrati, Csanad Z.
Hickson, Ian D.
Brown, Grant W.
BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity
title BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity
title_full BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity
title_fullStr BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity
title_full_unstemmed BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity
title_short BLM and RMI1 Alleviate RPA Inhibition of TopoIIIα Decatenase Activity
title_sort blm and rmi1 alleviate rpa inhibition of topoiiiα decatenase activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401101/
https://www.ncbi.nlm.nih.gov/pubmed/22911760
http://dx.doi.org/10.1371/journal.pone.0041208
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