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Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein
Streptococcus suis serotype 2 (S. suis 2) is an important pathogen, responsible for diverse diseases in swine and humans. To obtain a S. suis 2 strain that can be tracked in vitro and in vivo, we constructed the Egfp-HA9801 recombinant S. suis 2 strain with egfp and spc(r) genes inserted via homolog...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401235/ https://www.ncbi.nlm.nih.gov/pubmed/22911688 http://dx.doi.org/10.1371/journal.pone.0039697 |
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author | Chen, Tao Huang, Qin Li, Zhaolong Zhang, Wei Lu, Chengping Yao, Huochun |
author_facet | Chen, Tao Huang, Qin Li, Zhaolong Zhang, Wei Lu, Chengping Yao, Huochun |
author_sort | Chen, Tao |
collection | PubMed |
description | Streptococcus suis serotype 2 (S. suis 2) is an important pathogen, responsible for diverse diseases in swine and humans. To obtain a S. suis 2 strain that can be tracked in vitro and in vivo, we constructed the Egfp-HA9801 recombinant S. suis 2 strain with egfp and spc(r) genes inserted via homologous recombination. To assess the effects of the egfp and spc(r) genes in HA9801, the biochemical characteristics, growth features and virulence in Balb/C mice were compared between the recombinant and the parent HA9801 strain. We detected the EGFP expression from Egfp-HA9801 by epifluorescence microscopy. The results showed that the biochemical characterization and growth features of the Egfp-HA9801 recombinant were highly similar to that of the parent HA9801. We did not find significant differences in lethality (50% lethal dose), morbidity and mortality between the two strains. Furthermore, the bacterial counts in each various tissues of Egfp-HA9801-infected mice displayed similar dynamic compared with the HA9801-infected mice. Our results also showed that the Egfp-HA9801 cells grown at 37°C for 36 h displayed greater green fluorescence signals than the cells grown at 28°C for 36 h and 37°C for 24 h. The fluorescence in the tissue cryosections of Egfp-HA9801-injected mice was also stronger than that of the HA9801 group. Together, these results indicate that the egfp and spc(r) insertions into the Egfp-HA9801 recombinant did not significantly change the virulence when compared with HA980, and this EGFP labeled strain can be used for future S. suis 2 pathogenesis research. |
format | Online Article Text |
id | pubmed-3401235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34012352012-07-30 Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein Chen, Tao Huang, Qin Li, Zhaolong Zhang, Wei Lu, Chengping Yao, Huochun PLoS One Research Article Streptococcus suis serotype 2 (S. suis 2) is an important pathogen, responsible for diverse diseases in swine and humans. To obtain a S. suis 2 strain that can be tracked in vitro and in vivo, we constructed the Egfp-HA9801 recombinant S. suis 2 strain with egfp and spc(r) genes inserted via homologous recombination. To assess the effects of the egfp and spc(r) genes in HA9801, the biochemical characteristics, growth features and virulence in Balb/C mice were compared between the recombinant and the parent HA9801 strain. We detected the EGFP expression from Egfp-HA9801 by epifluorescence microscopy. The results showed that the biochemical characterization and growth features of the Egfp-HA9801 recombinant were highly similar to that of the parent HA9801. We did not find significant differences in lethality (50% lethal dose), morbidity and mortality between the two strains. Furthermore, the bacterial counts in each various tissues of Egfp-HA9801-infected mice displayed similar dynamic compared with the HA9801-infected mice. Our results also showed that the Egfp-HA9801 cells grown at 37°C for 36 h displayed greater green fluorescence signals than the cells grown at 28°C for 36 h and 37°C for 24 h. The fluorescence in the tissue cryosections of Egfp-HA9801-injected mice was also stronger than that of the HA9801 group. Together, these results indicate that the egfp and spc(r) insertions into the Egfp-HA9801 recombinant did not significantly change the virulence when compared with HA980, and this EGFP labeled strain can be used for future S. suis 2 pathogenesis research. Public Library of Science 2012-07-20 /pmc/articles/PMC3401235/ /pubmed/22911688 http://dx.doi.org/10.1371/journal.pone.0039697 Text en Chen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Tao Huang, Qin Li, Zhaolong Zhang, Wei Lu, Chengping Yao, Huochun Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein |
title | Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein |
title_full | Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein |
title_fullStr | Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein |
title_full_unstemmed | Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein |
title_short | Construction and Characterization of a Streptococcus suis Serotype 2 Recombinant Expressing Enhanced Green Fluorescent Protein |
title_sort | construction and characterization of a streptococcus suis serotype 2 recombinant expressing enhanced green fluorescent protein |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401235/ https://www.ncbi.nlm.nih.gov/pubmed/22911688 http://dx.doi.org/10.1371/journal.pone.0039697 |
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