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Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines
Bone marrow metastases are formed in the late phases of prostate cancer disease. Stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) are present in the microenvironment of the bone marrow and play a vital role in cell biology therein. The present study was to investigate the inf...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401500/ https://www.ncbi.nlm.nih.gov/pubmed/22252524 http://dx.doi.org/10.1007/s13277-012-0325-3 |
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author | Ma, Yuanyuan Liang, Dongming Liu, Jian Axcrona, Karol Kvalheim, Gunnar Giercksky, Karl-Erik Nesland, Jahn M. Suo, Zhenhe |
author_facet | Ma, Yuanyuan Liang, Dongming Liu, Jian Axcrona, Karol Kvalheim, Gunnar Giercksky, Karl-Erik Nesland, Jahn M. Suo, Zhenhe |
author_sort | Ma, Yuanyuan |
collection | PubMed |
description | Bone marrow metastases are formed in the late phases of prostate cancer disease. Stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) are present in the microenvironment of the bone marrow and play a vital role in cell biology therein. The present study was to investigate the influence of SCF and G-CSF on stem-like properties in prostate cancer cell lines. Upon stimulation with SCF or G-CSF, higher levels of CD117, ABCG2, and CD44 were observed in PC-3 and DU145 cells examined by flow cytometry. Simultaneously, the expressions of Oct3/4 and Nanog were upregulated. Moreover, quantitative real-time PCR verified that the increased Nanog under the stimulations was mostly derived from NANOGP8. In parallel with the increasing expressions of these proteins, higher colony and sphere formation efficiencies were seen in these cells in response to the cytokine stimulations. Furthermore, a synergistic effect of SCF and G-CSF on colony and sphere formations and ABCG2 expression was disclosed. Our results indicate a favorable bone marrow niche for prostate cancer cells where higher levels of cell stemness are maintained at least partly by the cytokines SCF and G-CSF. |
format | Online Article Text |
id | pubmed-3401500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-34015002012-07-23 Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines Ma, Yuanyuan Liang, Dongming Liu, Jian Axcrona, Karol Kvalheim, Gunnar Giercksky, Karl-Erik Nesland, Jahn M. Suo, Zhenhe Tumour Biol Research Article Bone marrow metastases are formed in the late phases of prostate cancer disease. Stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) are present in the microenvironment of the bone marrow and play a vital role in cell biology therein. The present study was to investigate the influence of SCF and G-CSF on stem-like properties in prostate cancer cell lines. Upon stimulation with SCF or G-CSF, higher levels of CD117, ABCG2, and CD44 were observed in PC-3 and DU145 cells examined by flow cytometry. Simultaneously, the expressions of Oct3/4 and Nanog were upregulated. Moreover, quantitative real-time PCR verified that the increased Nanog under the stimulations was mostly derived from NANOGP8. In parallel with the increasing expressions of these proteins, higher colony and sphere formation efficiencies were seen in these cells in response to the cytokine stimulations. Furthermore, a synergistic effect of SCF and G-CSF on colony and sphere formations and ABCG2 expression was disclosed. Our results indicate a favorable bone marrow niche for prostate cancer cells where higher levels of cell stemness are maintained at least partly by the cytokines SCF and G-CSF. Springer Netherlands 2012-01-18 /pmc/articles/PMC3401500/ /pubmed/22252524 http://dx.doi.org/10.1007/s13277-012-0325-3 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Research Article Ma, Yuanyuan Liang, Dongming Liu, Jian Axcrona, Karol Kvalheim, Gunnar Giercksky, Karl-Erik Nesland, Jahn M. Suo, Zhenhe Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines |
title | Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines |
title_full | Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines |
title_fullStr | Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines |
title_full_unstemmed | Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines |
title_short | Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines |
title_sort | synergistic effect of scf and g-csf on stem-like properties in prostate cancer cell lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401500/ https://www.ncbi.nlm.nih.gov/pubmed/22252524 http://dx.doi.org/10.1007/s13277-012-0325-3 |
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