Efficient Recombinant Production in Mammalian Cells Using a Novel IR/MAR Gene Amplification Method

We previously found that plasmids bearing a mammalian replication initiation region (IR) and a nuclear matrix attachment region (MAR) efficiently initiate gene amplification and spontaneously increase their copy numbers in animal cells. In this study, this novel method was applied to the establishme...

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Detalles Bibliográficos
Autores principales: Araki, Yoshio, Hamafuji, Tetsuro, Noguchi, Chiemi, Shimizu, Noriaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3402416/
https://www.ncbi.nlm.nih.gov/pubmed/22844523
http://dx.doi.org/10.1371/journal.pone.0041787

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3402416/
https://www.ncbi.nlm.nih.gov/pubmed/22844523
http://dx.doi.org/10.1371/journal.pone.0041787

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