Cargando…

Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels

Vascular tissue engineering focuses on the replacement of diseased small-diameter blood vessels with a diameter less than 6 mm for which adequate substitutes still do not exist. One approach to vascular tissue engineering is to culture vascular cells on a scaffold in a bioreactor. The bioreactor est...

Descripción completa

Detalles Bibliográficos
Autores principales: Meghezi, Sébastien, Couet, Frédéric, Chevallier, Pascale, Mantovani, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3403400/
https://www.ncbi.nlm.nih.gov/pubmed/22844285
http://dx.doi.org/10.1155/2012/319290
_version_ 1782238883768958976
author Meghezi, Sébastien
Couet, Frédéric
Chevallier, Pascale
Mantovani, Diego
author_facet Meghezi, Sébastien
Couet, Frédéric
Chevallier, Pascale
Mantovani, Diego
author_sort Meghezi, Sébastien
collection PubMed
description Vascular tissue engineering focuses on the replacement of diseased small-diameter blood vessels with a diameter less than 6 mm for which adequate substitutes still do not exist. One approach to vascular tissue engineering is to culture vascular cells on a scaffold in a bioreactor. The bioreactor establishes pseudophysiological conditions for culture (medium culture, 37°C, mechanical stimulation). Collagen gels are widely used as scaffolds for tissue regeneration due to their biological properties; however, they exhibit low mechanical properties. Mechanical characterization of these scaffolds requires establishing the conditions of testing in regard to the conditions set in the bioreactor. The effects of different parameters used during mechanical testing on the collagen gels were evaluated in terms of mechanical and viscoelastic properties. Thus, a factorial experiment was adopted, and three relevant factors were considered: temperature (23°C or 37°C), hydration (aqueous saline solution or air), and mechanical preconditioning (with or without). Statistical analyses showed significant effects of these factors on the mechanical properties which were assessed by tensile tests as well as stress relaxation tests. The last tests provide a more consistent understanding of the gels' viscoelastic properties. Therefore, performing mechanical analyses on hydrogels requires setting an adequate environment in terms of temperature and aqueous saline solution as well as choosing the adequate test.
format Online
Article
Text
id pubmed-3403400
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-34034002012-07-27 Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels Meghezi, Sébastien Couet, Frédéric Chevallier, Pascale Mantovani, Diego Int J Biomater Research Article Vascular tissue engineering focuses on the replacement of diseased small-diameter blood vessels with a diameter less than 6 mm for which adequate substitutes still do not exist. One approach to vascular tissue engineering is to culture vascular cells on a scaffold in a bioreactor. The bioreactor establishes pseudophysiological conditions for culture (medium culture, 37°C, mechanical stimulation). Collagen gels are widely used as scaffolds for tissue regeneration due to their biological properties; however, they exhibit low mechanical properties. Mechanical characterization of these scaffolds requires establishing the conditions of testing in regard to the conditions set in the bioreactor. The effects of different parameters used during mechanical testing on the collagen gels were evaluated in terms of mechanical and viscoelastic properties. Thus, a factorial experiment was adopted, and three relevant factors were considered: temperature (23°C or 37°C), hydration (aqueous saline solution or air), and mechanical preconditioning (with or without). Statistical analyses showed significant effects of these factors on the mechanical properties which were assessed by tensile tests as well as stress relaxation tests. The last tests provide a more consistent understanding of the gels' viscoelastic properties. Therefore, performing mechanical analyses on hydrogels requires setting an adequate environment in terms of temperature and aqueous saline solution as well as choosing the adequate test. Hindawi Publishing Corporation 2012 2012-07-12 /pmc/articles/PMC3403400/ /pubmed/22844285 http://dx.doi.org/10.1155/2012/319290 Text en Copyright © 2012 Sébastien Meghezi et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Meghezi, Sébastien
Couet, Frédéric
Chevallier, Pascale
Mantovani, Diego
Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels
title Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels
title_full Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels
title_fullStr Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels
title_full_unstemmed Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels
title_short Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels
title_sort effects of a pseudophysiological environment on the elastic and viscoelastic properties of collagen gels
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3403400/
https://www.ncbi.nlm.nih.gov/pubmed/22844285
http://dx.doi.org/10.1155/2012/319290
work_keys_str_mv AT meghezisebastien effectsofapseudophysiologicalenvironmentontheelasticandviscoelasticpropertiesofcollagengels
AT couetfrederic effectsofapseudophysiologicalenvironmentontheelasticandviscoelasticpropertiesofcollagengels
AT chevallierpascale effectsofapseudophysiologicalenvironmentontheelasticandviscoelasticpropertiesofcollagengels
AT mantovanidiego effectsofapseudophysiologicalenvironmentontheelasticandviscoelasticpropertiesofcollagengels