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Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs

The diversity of fecal methanogens of Erhualian (obese type) and Landrace (lean type) pigs was examined using separate 16S rRNA gene libraries for each breed. A total of 763 clones were analyzed; 381 from the Erhualian library and 382 from the Landrace library were identified belonging to the genus...

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Autores principales: Luo, Yu-heng, Su, Yong, Wright, André-Denis G., Zhang, Ling-li, Smidt, Hauke, Zhu, Wei-yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3403511/
https://www.ncbi.nlm.nih.gov/pubmed/22844227
http://dx.doi.org/10.1155/2012/605289
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author Luo, Yu-heng
Su, Yong
Wright, André-Denis G.
Zhang, Ling-li
Smidt, Hauke
Zhu, Wei-yun
author_facet Luo, Yu-heng
Su, Yong
Wright, André-Denis G.
Zhang, Ling-li
Smidt, Hauke
Zhu, Wei-yun
author_sort Luo, Yu-heng
collection PubMed
description The diversity of fecal methanogens of Erhualian (obese type) and Landrace (lean type) pigs was examined using separate 16S rRNA gene libraries for each breed. A total of 763 clones were analyzed; 381 from the Erhualian library and 382 from the Landrace library were identified belonging to the genus Methanobrevibacter. Others were identified belonging to the genus Methanosphaera. The two libraries showed significant differences in diversity (P < 0.05) and composition (P < 0.0001). Only two operational taxonomic units (OTUs) were found in both libraries, whereas six OTUs were found only in the Erhualian library and 23 OTUs were found only in the Landrace library. Real-time PCR showed that the abundance of fecal methanogens in Landrace pigs was significantly higher than that in Erhualian pigs (P < 0.05). Results showed that the Landrace pig (lean) harbored a greater diversity and higher numbers of methanogen mcrA gene copies than the Erhualian pig (obese). These differences may be related to the fatness or leanness in these two pig breeds. The results provide new leads for further investigations on the fat storage of pigs or even humans.
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spelling pubmed-34035112012-07-27 Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs Luo, Yu-heng Su, Yong Wright, André-Denis G. Zhang, Ling-li Smidt, Hauke Zhu, Wei-yun Archaea Research Article The diversity of fecal methanogens of Erhualian (obese type) and Landrace (lean type) pigs was examined using separate 16S rRNA gene libraries for each breed. A total of 763 clones were analyzed; 381 from the Erhualian library and 382 from the Landrace library were identified belonging to the genus Methanobrevibacter. Others were identified belonging to the genus Methanosphaera. The two libraries showed significant differences in diversity (P < 0.05) and composition (P < 0.0001). Only two operational taxonomic units (OTUs) were found in both libraries, whereas six OTUs were found only in the Erhualian library and 23 OTUs were found only in the Landrace library. Real-time PCR showed that the abundance of fecal methanogens in Landrace pigs was significantly higher than that in Erhualian pigs (P < 0.05). Results showed that the Landrace pig (lean) harbored a greater diversity and higher numbers of methanogen mcrA gene copies than the Erhualian pig (obese). These differences may be related to the fatness or leanness in these two pig breeds. The results provide new leads for further investigations on the fat storage of pigs or even humans. Hindawi Publishing Corporation 2012-07-16 /pmc/articles/PMC3403511/ /pubmed/22844227 http://dx.doi.org/10.1155/2012/605289 Text en Copyright © 2012 Yu-heng Luo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Luo, Yu-heng
Su, Yong
Wright, André-Denis G.
Zhang, Ling-li
Smidt, Hauke
Zhu, Wei-yun
Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs
title Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs
title_full Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs
title_fullStr Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs
title_full_unstemmed Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs
title_short Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs
title_sort lean breed landrace pigs harbor fecal methanogens at higher diversity and density than obese breed erhualian pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3403511/
https://www.ncbi.nlm.nih.gov/pubmed/22844227
http://dx.doi.org/10.1155/2012/605289
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