Cargando…

A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth

BACKGROUND: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) is a relatively non-toxic selective AhR modulator. This study was to detect the effects of DIM on gastric cancer cell growth. METHODS: Gastr...

Descripción completa

Detalles Bibliográficos
Autores principales: Yin, Xiao-Fei, Chen, Jie, Mao, Wei, Wang, Yu-Hong, Chen, Min-Hu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3403951/
https://www.ncbi.nlm.nih.gov/pubmed/22592002
http://dx.doi.org/10.1186/1756-9966-31-46
_version_ 1782238954383212544
author Yin, Xiao-Fei
Chen, Jie
Mao, Wei
Wang, Yu-Hong
Chen, Min-Hu
author_facet Yin, Xiao-Fei
Chen, Jie
Mao, Wei
Wang, Yu-Hong
Chen, Min-Hu
author_sort Yin, Xiao-Fei
collection PubMed
description BACKGROUND: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) is a relatively non-toxic selective AhR modulator. This study was to detect the effects of DIM on gastric cancer cell growth. METHODS: Gastric cancer cell SGC7901 was treated with DIM at different concentrations (0,10,20,30,40,50 μmol/L) with or without an AhR antagonist, resveratrol. The expression of AhR and Cytochrome P4501A1 (CYP1A1), a classic target gene of AhR pathway, were detected by RT-PCR and Western blot; cell viability was measured by MTT assay, and the changes in cell cycle and apoptosis were analyzed by flow cytometry. RESULTS: RT-PCR and western-blot showed that with the increase of the concentration of DIM, AhR protein gradually decreased and CYP1A1 expression increased, suggesting that DIM activated the AhR pathway and caused the translocation of AhR from cytoplasm to nucleus. MTT assay indicated that the viability of SGC7901 cells was significantly decreased in a concentration- and time-dependent manner after DIM treatment and this could be partially reversed by resveratrol. Flow cytometry analysis showed that DIM arrested cell cycle in G1 phase and induced cell apoptosis. CONCLUSION: Selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits SGC7901 cell proliferation by inducing apoptosis and delaying cell cycle progression. AhR may be a potential therapeutic target for gastric cancer treatment.
format Online
Article
Text
id pubmed-3403951
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-34039512012-07-25 A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth Yin, Xiao-Fei Chen, Jie Mao, Wei Wang, Yu-Hong Chen, Min-Hu J Exp Clin Cancer Res Research BACKGROUND: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) is a relatively non-toxic selective AhR modulator. This study was to detect the effects of DIM on gastric cancer cell growth. METHODS: Gastric cancer cell SGC7901 was treated with DIM at different concentrations (0,10,20,30,40,50 μmol/L) with or without an AhR antagonist, resveratrol. The expression of AhR and Cytochrome P4501A1 (CYP1A1), a classic target gene of AhR pathway, were detected by RT-PCR and Western blot; cell viability was measured by MTT assay, and the changes in cell cycle and apoptosis were analyzed by flow cytometry. RESULTS: RT-PCR and western-blot showed that with the increase of the concentration of DIM, AhR protein gradually decreased and CYP1A1 expression increased, suggesting that DIM activated the AhR pathway and caused the translocation of AhR from cytoplasm to nucleus. MTT assay indicated that the viability of SGC7901 cells was significantly decreased in a concentration- and time-dependent manner after DIM treatment and this could be partially reversed by resveratrol. Flow cytometry analysis showed that DIM arrested cell cycle in G1 phase and induced cell apoptosis. CONCLUSION: Selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits SGC7901 cell proliferation by inducing apoptosis and delaying cell cycle progression. AhR may be a potential therapeutic target for gastric cancer treatment. BioMed Central 2012-05-16 /pmc/articles/PMC3403951/ /pubmed/22592002 http://dx.doi.org/10.1186/1756-9966-31-46 Text en Copyright ©2012 Yin et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Yin, Xiao-Fei
Chen, Jie
Mao, Wei
Wang, Yu-Hong
Chen, Min-Hu
A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth
title A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth
title_full A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth
title_fullStr A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth
title_full_unstemmed A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth
title_short A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth
title_sort selective aryl hydrocarbon receptor modulator 3,3'-diindolylmethane inhibits gastric cancer cell growth
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3403951/
https://www.ncbi.nlm.nih.gov/pubmed/22592002
http://dx.doi.org/10.1186/1756-9966-31-46
work_keys_str_mv AT yinxiaofei aselectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT chenjie aselectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT maowei aselectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT wangyuhong aselectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT chenminhu aselectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT yinxiaofei selectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT chenjie selectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT maowei selectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT wangyuhong selectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth
AT chenminhu selectivearylhydrocarbonreceptormodulator33diindolylmethaneinhibitsgastriccancercellgrowth