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Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep

The Jaagsiekte sheep retrovirus exJSRV and its endogenous counterpart enJSRV co-exist in sheep. exJSRV, a betaretrovirus, is the etiological agent of ovine pulmonary adenocarcinoma, and it has been demonstrated in vitro that an enJSRV Gag variant bearing the R-to-W amino acid change at position 21 w...

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Autores principales: Viginier, Barbara, Dolmazon, Christine, Lantier, Isabelle, Lantier, Frédéric, Archer, Fabienne, Leroux, Caroline, Terzian, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3404005/
https://www.ncbi.nlm.nih.gov/pubmed/22911867
http://dx.doi.org/10.1371/journal.pone.0041965
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author Viginier, Barbara
Dolmazon, Christine
Lantier, Isabelle
Lantier, Frédéric
Archer, Fabienne
Leroux, Caroline
Terzian, Christophe
author_facet Viginier, Barbara
Dolmazon, Christine
Lantier, Isabelle
Lantier, Frédéric
Archer, Fabienne
Leroux, Caroline
Terzian, Christophe
author_sort Viginier, Barbara
collection PubMed
description The Jaagsiekte sheep retrovirus exJSRV and its endogenous counterpart enJSRV co-exist in sheep. exJSRV, a betaretrovirus, is the etiological agent of ovine pulmonary adenocarcinoma, and it has been demonstrated in vitro that an enJSRV Gag variant bearing the R-to-W amino acid change at position 21 was able to block exJSRV budding from the cells, providing a potential protective role for the host. In this work, we developed a fast mutation detection assay based on the oligo ligation assay (OLA) that permits the quantification of the relative proportions of the R21 and W21 Gag variants present in individual genomes and in cDNA obtained from normal and exJSRV-induced lung tumors. We have shown that the W21/R21 ratio is variable within and between breeds. We also describe for the first time that putative protecting enJSRV variants were expressed in alveolar type II cells (AECII), the major target of exJSRV.
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spelling pubmed-34040052012-07-30 Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep Viginier, Barbara Dolmazon, Christine Lantier, Isabelle Lantier, Frédéric Archer, Fabienne Leroux, Caroline Terzian, Christophe PLoS One Research Article The Jaagsiekte sheep retrovirus exJSRV and its endogenous counterpart enJSRV co-exist in sheep. exJSRV, a betaretrovirus, is the etiological agent of ovine pulmonary adenocarcinoma, and it has been demonstrated in vitro that an enJSRV Gag variant bearing the R-to-W amino acid change at position 21 was able to block exJSRV budding from the cells, providing a potential protective role for the host. In this work, we developed a fast mutation detection assay based on the oligo ligation assay (OLA) that permits the quantification of the relative proportions of the R21 and W21 Gag variants present in individual genomes and in cDNA obtained from normal and exJSRV-induced lung tumors. We have shown that the W21/R21 ratio is variable within and between breeds. We also describe for the first time that putative protecting enJSRV variants were expressed in alveolar type II cells (AECII), the major target of exJSRV. Public Library of Science 2012-07-24 /pmc/articles/PMC3404005/ /pubmed/22911867 http://dx.doi.org/10.1371/journal.pone.0041965 Text en Viginier et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Viginier, Barbara
Dolmazon, Christine
Lantier, Isabelle
Lantier, Frédéric
Archer, Fabienne
Leroux, Caroline
Terzian, Christophe
Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep
title Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep
title_full Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep
title_fullStr Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep
title_full_unstemmed Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep
title_short Copy Number Variation and Differential Expression of a Protective Endogenous Retrovirus in Sheep
title_sort copy number variation and differential expression of a protective endogenous retrovirus in sheep
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3404005/
https://www.ncbi.nlm.nih.gov/pubmed/22911867
http://dx.doi.org/10.1371/journal.pone.0041965
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