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Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract

In fertile women, the endometrium undergoes regular cycles of tissue build-up and regression. It is likely that uterine stem cells are involved in this remarkable turn over. The main goal of our current investigations was to identify slow-cycling (quiescent) endometrial stem cells by means of a puls...

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Autores principales: Wang, Yongyi, Sacchetti, Andrea, van Dijk, Matthijs R., van der Zee, Marten, van der Horst, Paul H., Joosten, Rosalie, Burger, Curt W., Grootegoed, J. Anton, Blok, Leen J., Fodde, Riccardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3404087/
https://www.ncbi.nlm.nih.gov/pubmed/22848396
http://dx.doi.org/10.1371/journal.pone.0040691
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author Wang, Yongyi
Sacchetti, Andrea
van Dijk, Matthijs R.
van der Zee, Marten
van der Horst, Paul H.
Joosten, Rosalie
Burger, Curt W.
Grootegoed, J. Anton
Blok, Leen J.
Fodde, Riccardo
author_facet Wang, Yongyi
Sacchetti, Andrea
van Dijk, Matthijs R.
van der Zee, Marten
van der Horst, Paul H.
Joosten, Rosalie
Burger, Curt W.
Grootegoed, J. Anton
Blok, Leen J.
Fodde, Riccardo
author_sort Wang, Yongyi
collection PubMed
description In fertile women, the endometrium undergoes regular cycles of tissue build-up and regression. It is likely that uterine stem cells are involved in this remarkable turn over. The main goal of our current investigations was to identify slow-cycling (quiescent) endometrial stem cells by means of a pulse-chase approach to selectively earmark, prospectively isolate, and characterize label-retaining cells (LRCs). To this aim, transgenic mice expressing histone2B-GFP (H2B-GFP) in a Tet-inducible fashion were administered doxycycline (pulse) which was thereafter withdrawn from the drinking water (chase). Over time, dividing cells progressively loose GFP signal whereas infrequently dividing cells retain H2B-GFP expression. We evaluated H2B-GFP retaining cells at different chase time points and identified long-term (LT; >12 weeks) LRCs. The LT-LRCs are negative for estrogen receptor-α and express low levels of progesterone receptors. LRCs sorted by FACS are able to form spheroids capable of self-renewal and differentiation. Upon serum stimulation spheroid cells are induced to differentiate and form glandular structures which express markers of mature Műllerian epithelial cells. Overall, the results indicate that quiescent cells located in the distal oviduct have stem-like properties and can differentiate into distinct cell lineages specific of endometrium, proximal and distal oviduct. Future lineage-tracing studies will elucidate the role played by these cells in homeostasis, tissue injury and cancer of the female reproductive tract in the mouse and eventually in man.
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spelling pubmed-34040872012-07-30 Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract Wang, Yongyi Sacchetti, Andrea van Dijk, Matthijs R. van der Zee, Marten van der Horst, Paul H. Joosten, Rosalie Burger, Curt W. Grootegoed, J. Anton Blok, Leen J. Fodde, Riccardo PLoS One Research Article In fertile women, the endometrium undergoes regular cycles of tissue build-up and regression. It is likely that uterine stem cells are involved in this remarkable turn over. The main goal of our current investigations was to identify slow-cycling (quiescent) endometrial stem cells by means of a pulse-chase approach to selectively earmark, prospectively isolate, and characterize label-retaining cells (LRCs). To this aim, transgenic mice expressing histone2B-GFP (H2B-GFP) in a Tet-inducible fashion were administered doxycycline (pulse) which was thereafter withdrawn from the drinking water (chase). Over time, dividing cells progressively loose GFP signal whereas infrequently dividing cells retain H2B-GFP expression. We evaluated H2B-GFP retaining cells at different chase time points and identified long-term (LT; >12 weeks) LRCs. The LT-LRCs are negative for estrogen receptor-α and express low levels of progesterone receptors. LRCs sorted by FACS are able to form spheroids capable of self-renewal and differentiation. Upon serum stimulation spheroid cells are induced to differentiate and form glandular structures which express markers of mature Műllerian epithelial cells. Overall, the results indicate that quiescent cells located in the distal oviduct have stem-like properties and can differentiate into distinct cell lineages specific of endometrium, proximal and distal oviduct. Future lineage-tracing studies will elucidate the role played by these cells in homeostasis, tissue injury and cancer of the female reproductive tract in the mouse and eventually in man. Public Library of Science 2012-07-24 /pmc/articles/PMC3404087/ /pubmed/22848396 http://dx.doi.org/10.1371/journal.pone.0040691 Text en Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Yongyi
Sacchetti, Andrea
van Dijk, Matthijs R.
van der Zee, Marten
van der Horst, Paul H.
Joosten, Rosalie
Burger, Curt W.
Grootegoed, J. Anton
Blok, Leen J.
Fodde, Riccardo
Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract
title Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract
title_full Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract
title_fullStr Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract
title_full_unstemmed Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract
title_short Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract
title_sort identification of quiescent, stem-like cells in the distal female reproductive tract
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3404087/
https://www.ncbi.nlm.nih.gov/pubmed/22848396
http://dx.doi.org/10.1371/journal.pone.0040691
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