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Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation
The development of autoantibodies is a hallmark of systemic lupus erythematosus (SLE). SLE serum can induce monocyte differentiation into dendritic cells (DCs) in a type I IFN–dependent manner. Such SLE-DCs activate T cells, but whether they promote B cell responses is not known. In this study, we d...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405503/ https://www.ncbi.nlm.nih.gov/pubmed/22689824 http://dx.doi.org/10.1084/jem.20111644 |
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author | Joo, HyeMee Coquery, Christine Xue, Yaming Gayet, Ingrid Dillon, Stacey R. Punaro, Marilynn Zurawski, Gerard Banchereau, Jacques Pascual, Virginia Oh, SangKon |
author_facet | Joo, HyeMee Coquery, Christine Xue, Yaming Gayet, Ingrid Dillon, Stacey R. Punaro, Marilynn Zurawski, Gerard Banchereau, Jacques Pascual, Virginia Oh, SangKon |
author_sort | Joo, HyeMee |
collection | PubMed |
description | The development of autoantibodies is a hallmark of systemic lupus erythematosus (SLE). SLE serum can induce monocyte differentiation into dendritic cells (DCs) in a type I IFN–dependent manner. Such SLE-DCs activate T cells, but whether they promote B cell responses is not known. In this study, we demonstrate that SLE-DCs can efficiently stimulate naive and memory B cells to differentiate into IgG- and IgA-plasmablasts (PBs) resembling those found in the blood of SLE patients. SLE-DC–mediated IgG-PB differentiation is dependent on B cell–activating factor (BAFF) and IL-10, whereas IgA-PB differentiation is dependent on a proliferation-inducing ligand (APRIL). Importantly, SLE-DCs express CD138 and trans-present CD138-bound APRIL to B cells, leading to the induction of IgA switching and PB differentiation in an IFN-α–independent manner. We further found that this mechanism of providing B cell help is relevant in vivo, as CD138-bound APRIL is expressed on blood monocytes from active SLE patients. Collectively, our study suggests that a direct myeloid DC–B cell interplay might contribute to the pathogenesis of SLE. |
format | Online Article Text |
id | pubmed-3405503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34055032013-01-02 Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation Joo, HyeMee Coquery, Christine Xue, Yaming Gayet, Ingrid Dillon, Stacey R. Punaro, Marilynn Zurawski, Gerard Banchereau, Jacques Pascual, Virginia Oh, SangKon J Exp Med Article The development of autoantibodies is a hallmark of systemic lupus erythematosus (SLE). SLE serum can induce monocyte differentiation into dendritic cells (DCs) in a type I IFN–dependent manner. Such SLE-DCs activate T cells, but whether they promote B cell responses is not known. In this study, we demonstrate that SLE-DCs can efficiently stimulate naive and memory B cells to differentiate into IgG- and IgA-plasmablasts (PBs) resembling those found in the blood of SLE patients. SLE-DC–mediated IgG-PB differentiation is dependent on B cell–activating factor (BAFF) and IL-10, whereas IgA-PB differentiation is dependent on a proliferation-inducing ligand (APRIL). Importantly, SLE-DCs express CD138 and trans-present CD138-bound APRIL to B cells, leading to the induction of IgA switching and PB differentiation in an IFN-α–independent manner. We further found that this mechanism of providing B cell help is relevant in vivo, as CD138-bound APRIL is expressed on blood monocytes from active SLE patients. Collectively, our study suggests that a direct myeloid DC–B cell interplay might contribute to the pathogenesis of SLE. The Rockefeller University Press 2012-07-02 /pmc/articles/PMC3405503/ /pubmed/22689824 http://dx.doi.org/10.1084/jem.20111644 Text en © 2012 Joo et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Article Joo, HyeMee Coquery, Christine Xue, Yaming Gayet, Ingrid Dillon, Stacey R. Punaro, Marilynn Zurawski, Gerard Banchereau, Jacques Pascual, Virginia Oh, SangKon Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation |
title | Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation |
title_full | Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation |
title_fullStr | Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation |
title_full_unstemmed | Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation |
title_short | Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation |
title_sort | serum from patients with sle instructs monocytes to promote igg and iga plasmablast differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405503/ https://www.ncbi.nlm.nih.gov/pubmed/22689824 http://dx.doi.org/10.1084/jem.20111644 |
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