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Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration
The purpose of this study was to synthesize a self-setting bioactive cement by incorporation of wollastonite nanofibers (WNFs) into calcium phosphate cement (CPC). The composition, morphology, setting time, compressive strength, hydrophilicity, and degradation of WNF-doped CPC (wnf-CPC) were investi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405877/ https://www.ncbi.nlm.nih.gov/pubmed/22848181 http://dx.doi.org/10.2147/IJN.S32061 |
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author | Guo, Han Wei, Jie Song, Wenhua Zhang, Shan Yan, Yonggang Liu, Changsheng Xiao, Tiqiao |
author_facet | Guo, Han Wei, Jie Song, Wenhua Zhang, Shan Yan, Yonggang Liu, Changsheng Xiao, Tiqiao |
author_sort | Guo, Han |
collection | PubMed |
description | The purpose of this study was to synthesize a self-setting bioactive cement by incorporation of wollastonite nanofibers (WNFs) into calcium phosphate cement (CPC). The composition, morphology, setting time, compressive strength, hydrophilicity, and degradation of WNF-doped CPC (wnf-CPC) were investigated. Scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and inductively coupled plasma atomic emission spectroscopy were utilized. Additionally, methyl-thiazolyl-tetrazolium bromide assay, scanning electron microscopy, inductively coupled plasma atomic emission spectroscopy, and histological evaluation were used to study the cell and tissue responses to wnf-CPC, both in vitro and in vivo. The results confirmed that the addition of WNFs into CPC had no obvious effect on the setting time or the compressive strength of wnf-CPC, provided the WNF amount was not more than 10 wt%. However, the hydrophilicity and degradability of wnf-CPC were significantly improved by the addition of WNFs – this was because of the change of microstructure caused by the WNFs. The preferred dissolution of WNFs caused the formation of microporosity in wnf-CPC when soaked in tris hydrochloride solution. The microporosity enlarged the surface area of the wnf-CPC and so promoted degradation of the wnf-CPC when in contact with liquid. In addition, MG-63 cell attachment and proliferation on the wnf-CPC were superior to that on the CPC, indicating that incorporation of WNFs into CPC improved the biological properties for wnf-CPC. Following the implantation of wnf-CPC into bone defects of rabbits, histological evaluation showed that wnf-CPC enhanced the efficiency of new bone formation in comparison with CPC, indicating excellent biocompatibility and osteogenesis of wnf-CPC. In conclusion, wnf-CPC exhibited promising prospects in bone regeneration. |
format | Online Article Text |
id | pubmed-3405877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34058772012-07-30 Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration Guo, Han Wei, Jie Song, Wenhua Zhang, Shan Yan, Yonggang Liu, Changsheng Xiao, Tiqiao Int J Nanomedicine Original Research The purpose of this study was to synthesize a self-setting bioactive cement by incorporation of wollastonite nanofibers (WNFs) into calcium phosphate cement (CPC). The composition, morphology, setting time, compressive strength, hydrophilicity, and degradation of WNF-doped CPC (wnf-CPC) were investigated. Scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and inductively coupled plasma atomic emission spectroscopy were utilized. Additionally, methyl-thiazolyl-tetrazolium bromide assay, scanning electron microscopy, inductively coupled plasma atomic emission spectroscopy, and histological evaluation were used to study the cell and tissue responses to wnf-CPC, both in vitro and in vivo. The results confirmed that the addition of WNFs into CPC had no obvious effect on the setting time or the compressive strength of wnf-CPC, provided the WNF amount was not more than 10 wt%. However, the hydrophilicity and degradability of wnf-CPC were significantly improved by the addition of WNFs – this was because of the change of microstructure caused by the WNFs. The preferred dissolution of WNFs caused the formation of microporosity in wnf-CPC when soaked in tris hydrochloride solution. The microporosity enlarged the surface area of the wnf-CPC and so promoted degradation of the wnf-CPC when in contact with liquid. In addition, MG-63 cell attachment and proliferation on the wnf-CPC were superior to that on the CPC, indicating that incorporation of WNFs into CPC improved the biological properties for wnf-CPC. Following the implantation of wnf-CPC into bone defects of rabbits, histological evaluation showed that wnf-CPC enhanced the efficiency of new bone formation in comparison with CPC, indicating excellent biocompatibility and osteogenesis of wnf-CPC. In conclusion, wnf-CPC exhibited promising prospects in bone regeneration. Dove Medical Press 2012 2012-07-11 /pmc/articles/PMC3405877/ /pubmed/22848181 http://dx.doi.org/10.2147/IJN.S32061 Text en © 2012 Guo et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Guo, Han Wei, Jie Song, Wenhua Zhang, Shan Yan, Yonggang Liu, Changsheng Xiao, Tiqiao Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration |
title | Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration |
title_full | Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration |
title_fullStr | Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration |
title_full_unstemmed | Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration |
title_short | Wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration |
title_sort | wollastonite nanofiber–doped self-setting calcium phosphate bioactive cement for bone tissue regeneration |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405877/ https://www.ncbi.nlm.nih.gov/pubmed/22848181 http://dx.doi.org/10.2147/IJN.S32061 |
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