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Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation

Pollen-mediated transgene flow is a major concern for the production of genetically modified (GM) rice. Cleistogamy is a useful tool for preventing this form of gene flow. We previously identified the cleistogamous rice mutant superwoman1-cleistogamy (spw1-cls) and determined its molecular genetic m...

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Autores principales: Ohmori, Shinnosuke, Tabuchi, Hiroaki, Yatou, Osamu, Yoshida, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405965/
https://www.ncbi.nlm.nih.gov/pubmed/23136523
http://dx.doi.org/10.1270/jsbbs.62.124
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author Ohmori, Shinnosuke
Tabuchi, Hiroaki
Yatou, Osamu
Yoshida, Hitoshi
author_facet Ohmori, Shinnosuke
Tabuchi, Hiroaki
Yatou, Osamu
Yoshida, Hitoshi
author_sort Ohmori, Shinnosuke
collection PubMed
description Pollen-mediated transgene flow is a major concern for the production of genetically modified (GM) rice. Cleistogamy is a useful tool for preventing this form of gene flow. We previously identified the cleistogamous rice mutant superwoman1-cleistogamy (spw1-cls) and determined its molecular genetic mechanism. In the present study, we cultivated spw1-cls over five years to examine effects of cleistogamy on agronomic traits. Simultaneously, we cultivated cleistogamous backcross lines created by continuous backcrossing with “Yumeaoba” (a japonica cultivar) as the recurrent parent and by application of a DNA marker. In these experimental cultivations, spw1-cls and its backcross lines showed almost equal or slightly lower, but acceptable, agronomic traits compared with each control line. We also conducted natural crossing tests in paddy fields to assess the gene containment capability of spw1-cls. In a series of field experiments, there was no natural crossing between spw1-cls (pollen donor) and pollen recipient lines, but the wild-type donor and recipient lines were crossed. Thus, the cleistogamy of the spw1-cls mutation is able to inhibit natural crossing effectively, without significant loss of commercial benefits, such as yield. We conclude that spw1-cls cleistogamy is a practical tool for gene containment in GM rice cultivation.
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spelling pubmed-34059652012-11-07 Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation Ohmori, Shinnosuke Tabuchi, Hiroaki Yatou, Osamu Yoshida, Hitoshi Breed Sci Research Papers Pollen-mediated transgene flow is a major concern for the production of genetically modified (GM) rice. Cleistogamy is a useful tool for preventing this form of gene flow. We previously identified the cleistogamous rice mutant superwoman1-cleistogamy (spw1-cls) and determined its molecular genetic mechanism. In the present study, we cultivated spw1-cls over five years to examine effects of cleistogamy on agronomic traits. Simultaneously, we cultivated cleistogamous backcross lines created by continuous backcrossing with “Yumeaoba” (a japonica cultivar) as the recurrent parent and by application of a DNA marker. In these experimental cultivations, spw1-cls and its backcross lines showed almost equal or slightly lower, but acceptable, agronomic traits compared with each control line. We also conducted natural crossing tests in paddy fields to assess the gene containment capability of spw1-cls. In a series of field experiments, there was no natural crossing between spw1-cls (pollen donor) and pollen recipient lines, but the wild-type donor and recipient lines were crossed. Thus, the cleistogamy of the spw1-cls mutation is able to inhibit natural crossing effectively, without significant loss of commercial benefits, such as yield. We conclude that spw1-cls cleistogamy is a practical tool for gene containment in GM rice cultivation. Japanese Society of Breeding 2012-06-19 2012-06 /pmc/articles/PMC3405965/ /pubmed/23136523 http://dx.doi.org/10.1270/jsbbs.62.124 Text en Copyright © 2012 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Ohmori, Shinnosuke
Tabuchi, Hiroaki
Yatou, Osamu
Yoshida, Hitoshi
Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
title Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
title_full Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
title_fullStr Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
title_full_unstemmed Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
title_short Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
title_sort agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405965/
https://www.ncbi.nlm.nih.gov/pubmed/23136523
http://dx.doi.org/10.1270/jsbbs.62.124
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