Cargando…
The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3
In numerous species, the formation of meiotic crossovers is largely under the control of a group of proteins known as ZMM. Here, we identified a new ZMM protein, HEI10, a RING finger-containing protein that is well conserved among species. We show that HEI10 is structurally and functionally related...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405992/ https://www.ncbi.nlm.nih.gov/pubmed/22844245 http://dx.doi.org/10.1371/journal.pgen.1002799 |
_version_ | 1782239182875262976 |
---|---|
author | Chelysheva, Liudmila Vezon, Daniel Chambon, Aurélie Gendrot, Ghislaine Pereira, Lucie Lemhemdi, Afef Vrielynck, Nathalie Le Guin, Sylvia Novatchkova, Maria Grelon, Mathilde |
author_facet | Chelysheva, Liudmila Vezon, Daniel Chambon, Aurélie Gendrot, Ghislaine Pereira, Lucie Lemhemdi, Afef Vrielynck, Nathalie Le Guin, Sylvia Novatchkova, Maria Grelon, Mathilde |
author_sort | Chelysheva, Liudmila |
collection | PubMed |
description | In numerous species, the formation of meiotic crossovers is largely under the control of a group of proteins known as ZMM. Here, we identified a new ZMM protein, HEI10, a RING finger-containing protein that is well conserved among species. We show that HEI10 is structurally and functionally related to the yeast Zip3 ZMM and that it is absolutely required for class I crossover (CO) formation in Arabidopsis thaliana. Furthermore, we show that it is present as numerous foci on the chromosome axes and the synaptonemal complex central element until pachytene. Then, from pachytene to diakinesis, HEI10 is retained at a limited number of sites that correspond to class I COs, where it co-localises with MLH1. Assuming that HEI10 early staining represents an early selection of recombination intermediates to be channelled into the ZMM pathway, HEI10 would therefore draw a continuity between early chosen recombination intermediates and final class I COs. |
format | Online Article Text |
id | pubmed-3405992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34059922012-07-27 The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3 Chelysheva, Liudmila Vezon, Daniel Chambon, Aurélie Gendrot, Ghislaine Pereira, Lucie Lemhemdi, Afef Vrielynck, Nathalie Le Guin, Sylvia Novatchkova, Maria Grelon, Mathilde PLoS Genet Research Article In numerous species, the formation of meiotic crossovers is largely under the control of a group of proteins known as ZMM. Here, we identified a new ZMM protein, HEI10, a RING finger-containing protein that is well conserved among species. We show that HEI10 is structurally and functionally related to the yeast Zip3 ZMM and that it is absolutely required for class I crossover (CO) formation in Arabidopsis thaliana. Furthermore, we show that it is present as numerous foci on the chromosome axes and the synaptonemal complex central element until pachytene. Then, from pachytene to diakinesis, HEI10 is retained at a limited number of sites that correspond to class I COs, where it co-localises with MLH1. Assuming that HEI10 early staining represents an early selection of recombination intermediates to be channelled into the ZMM pathway, HEI10 would therefore draw a continuity between early chosen recombination intermediates and final class I COs. Public Library of Science 2012-07-26 /pmc/articles/PMC3405992/ /pubmed/22844245 http://dx.doi.org/10.1371/journal.pgen.1002799 Text en Chelysheva et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chelysheva, Liudmila Vezon, Daniel Chambon, Aurélie Gendrot, Ghislaine Pereira, Lucie Lemhemdi, Afef Vrielynck, Nathalie Le Guin, Sylvia Novatchkova, Maria Grelon, Mathilde The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3 |
title | The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3 |
title_full | The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3 |
title_fullStr | The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3 |
title_full_unstemmed | The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3 |
title_short | The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3 |
title_sort | arabidopsis hei10 is a new zmm protein related to zip3 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405992/ https://www.ncbi.nlm.nih.gov/pubmed/22844245 http://dx.doi.org/10.1371/journal.pgen.1002799 |
work_keys_str_mv | AT chelyshevaliudmila thearabidopsishei10isanewzmmproteinrelatedtozip3 AT vezondaniel thearabidopsishei10isanewzmmproteinrelatedtozip3 AT chambonaurelie thearabidopsishei10isanewzmmproteinrelatedtozip3 AT gendrotghislaine thearabidopsishei10isanewzmmproteinrelatedtozip3 AT pereiralucie thearabidopsishei10isanewzmmproteinrelatedtozip3 AT lemhemdiafef thearabidopsishei10isanewzmmproteinrelatedtozip3 AT vrielyncknathalie thearabidopsishei10isanewzmmproteinrelatedtozip3 AT leguinsylvia thearabidopsishei10isanewzmmproteinrelatedtozip3 AT novatchkovamaria thearabidopsishei10isanewzmmproteinrelatedtozip3 AT grelonmathilde thearabidopsishei10isanewzmmproteinrelatedtozip3 AT chelyshevaliudmila arabidopsishei10isanewzmmproteinrelatedtozip3 AT vezondaniel arabidopsishei10isanewzmmproteinrelatedtozip3 AT chambonaurelie arabidopsishei10isanewzmmproteinrelatedtozip3 AT gendrotghislaine arabidopsishei10isanewzmmproteinrelatedtozip3 AT pereiralucie arabidopsishei10isanewzmmproteinrelatedtozip3 AT lemhemdiafef arabidopsishei10isanewzmmproteinrelatedtozip3 AT vrielyncknathalie arabidopsishei10isanewzmmproteinrelatedtozip3 AT leguinsylvia arabidopsishei10isanewzmmproteinrelatedtozip3 AT novatchkovamaria arabidopsishei10isanewzmmproteinrelatedtozip3 AT grelonmathilde arabidopsishei10isanewzmmproteinrelatedtozip3 |