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Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro

Embryonic stem cells (ESCs) can be propagated in vitro on feeder layers of mouse STO fibroblast cells. The STO cells secrete several cytokines that are essential for ESCs to maintain their undifferentiated state. In this study, we found significant growth inhibition of mouse ESCs (mESCs) cultured on...

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Autores principales: Kim, Gu-Hee, Lee, Gong-Rak, Choi, Hyung-Im, Park, Neung-Hwa, Chung, Hun Taeg, Han, In-Seob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406291/
https://www.ncbi.nlm.nih.gov/pubmed/22595769
http://dx.doi.org/10.3858/emm.2012.44.7.052
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author Kim, Gu-Hee
Lee, Gong-Rak
Choi, Hyung-Im
Park, Neung-Hwa
Chung, Hun Taeg
Han, In-Seob
author_facet Kim, Gu-Hee
Lee, Gong-Rak
Choi, Hyung-Im
Park, Neung-Hwa
Chung, Hun Taeg
Han, In-Seob
author_sort Kim, Gu-Hee
collection PubMed
description Embryonic stem cells (ESCs) can be propagated in vitro on feeder layers of mouse STO fibroblast cells. The STO cells secrete several cytokines that are essential for ESCs to maintain their undifferentiated state. In this study, we found significant growth inhibition of mouse ESCs (mESCs) cultured on STO cells infected with adenovirus containing a dominant-negative mutant form of IκB (rAd-dnIκB). This blockage of the NF-κB signal pathway in STO cells led to a significant decrease in [(3)H]thymidine incorporation and colony formation of mESCs. Expression profile of cytokines secreted from the STO cells revealed an increase in the bone morphogenetic protein4 (BMP4) transcript level in the STO cells infected with adenoviral vector encoding dominant negative IκB (rAd-dnIκB). These results suggested that the NF-κB signaling pathway represses expression of BMP4 in STO feeder cells. Conditioned medium from the rAd-dnIκB-infected STO cells also significantly reduced the colony size of mESCs. Addition of BMP4 prevented colony formation of mESCs cultured in the conditioned medium. Our finding suggested that an excess of BMP4 in the conditioned medium also inhibits proliferation of mESCs.
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spelling pubmed-34062912012-08-04 Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro Kim, Gu-Hee Lee, Gong-Rak Choi, Hyung-Im Park, Neung-Hwa Chung, Hun Taeg Han, In-Seob Exp Mol Med Original Article Embryonic stem cells (ESCs) can be propagated in vitro on feeder layers of mouse STO fibroblast cells. The STO cells secrete several cytokines that are essential for ESCs to maintain their undifferentiated state. In this study, we found significant growth inhibition of mouse ESCs (mESCs) cultured on STO cells infected with adenovirus containing a dominant-negative mutant form of IκB (rAd-dnIκB). This blockage of the NF-κB signal pathway in STO cells led to a significant decrease in [(3)H]thymidine incorporation and colony formation of mESCs. Expression profile of cytokines secreted from the STO cells revealed an increase in the bone morphogenetic protein4 (BMP4) transcript level in the STO cells infected with adenoviral vector encoding dominant negative IκB (rAd-dnIκB). These results suggested that the NF-κB signaling pathway represses expression of BMP4 in STO feeder cells. Conditioned medium from the rAd-dnIκB-infected STO cells also significantly reduced the colony size of mESCs. Addition of BMP4 prevented colony formation of mESCs cultured in the conditioned medium. Our finding suggested that an excess of BMP4 in the conditioned medium also inhibits proliferation of mESCs. Korean Society for Biochemistry and Molecular Biology 2012-07-31 2012-05-17 /pmc/articles/PMC3406291/ /pubmed/22595769 http://dx.doi.org/10.3858/emm.2012.44.7.052 Text en Copyright © 2012 by the Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Gu-Hee
Lee, Gong-Rak
Choi, Hyung-Im
Park, Neung-Hwa
Chung, Hun Taeg
Han, In-Seob
Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
title Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
title_full Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
title_fullStr Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
title_full_unstemmed Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
title_short Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
title_sort overexpression of bone morphogenetic protein 4 in sto fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406291/
https://www.ncbi.nlm.nih.gov/pubmed/22595769
http://dx.doi.org/10.3858/emm.2012.44.7.052
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