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Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene

The peanut stunt virus (PSV) causes yield losses in soybean and reduced seed quality due to seed mottling. The objectives of this study were to determine the phenotypic reactions of soybean germplasms to inoculation with two PSV isolates (PSV-K, PSV-T), the inheritance of PSV resistance in soybean c...

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Autores principales: Saruta, Masayasu, Takada, Yoshitake, Kikuchi, Akio, Yamada, Tetsusya, Komatsu, Kunihiko, Sayama, Takashi, Ishimoto, Masao, Okabe, Akinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406795/
https://www.ncbi.nlm.nih.gov/pubmed/23136501
http://dx.doi.org/10.1270/jsbbs.61.625
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author Saruta, Masayasu
Takada, Yoshitake
Kikuchi, Akio
Yamada, Tetsusya
Komatsu, Kunihiko
Sayama, Takashi
Ishimoto, Masao
Okabe, Akinori
author_facet Saruta, Masayasu
Takada, Yoshitake
Kikuchi, Akio
Yamada, Tetsusya
Komatsu, Kunihiko
Sayama, Takashi
Ishimoto, Masao
Okabe, Akinori
author_sort Saruta, Masayasu
collection PubMed
description The peanut stunt virus (PSV) causes yield losses in soybean and reduced seed quality due to seed mottling. The objectives of this study were to determine the phenotypic reactions of soybean germplasms to inoculation with two PSV isolates (PSV-K, PSV-T), the inheritance of PSV resistance in soybean cultivars, and the locus of the PSV resistance gene. We investigated the PSV resistance of 132 soybean cultivars to both PSV isolates; of these, 73 cultivars exhibited resistance to both PSV isolates. Three resistant cultivars (Harosoy, Tsurunotamago 1 and Hyuga) were crossed with the susceptible cultivar Enrei. The crosses were evaluated in the F(1), F(2) and F(2:3) generations for their reactions to inoculation with the two PSV isolates. In an allelism test, we crossed Harosoy and Tsurunotamago 1 with the resistant cultivar Hyuga. The results revealed that PSV resistance in these cultivars is controlled by a single dominant gene at the same locus. We have proposed Rpsv1, as the name of the resistance gene in Hyuga. We also constructed a linkage map using recombinant inbred lines between Hyuga × Enrei using 176 SSR markers. We mapped Rpsv1 near the Satt435 locus on soybean chromosome 7.
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spelling pubmed-34067952012-11-07 Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene Saruta, Masayasu Takada, Yoshitake Kikuchi, Akio Yamada, Tetsusya Komatsu, Kunihiko Sayama, Takashi Ishimoto, Masao Okabe, Akinori Breed Sci Research Papers The peanut stunt virus (PSV) causes yield losses in soybean and reduced seed quality due to seed mottling. The objectives of this study were to determine the phenotypic reactions of soybean germplasms to inoculation with two PSV isolates (PSV-K, PSV-T), the inheritance of PSV resistance in soybean cultivars, and the locus of the PSV resistance gene. We investigated the PSV resistance of 132 soybean cultivars to both PSV isolates; of these, 73 cultivars exhibited resistance to both PSV isolates. Three resistant cultivars (Harosoy, Tsurunotamago 1 and Hyuga) were crossed with the susceptible cultivar Enrei. The crosses were evaluated in the F(1), F(2) and F(2:3) generations for their reactions to inoculation with the two PSV isolates. In an allelism test, we crossed Harosoy and Tsurunotamago 1 with the resistant cultivar Hyuga. The results revealed that PSV resistance in these cultivars is controlled by a single dominant gene at the same locus. We have proposed Rpsv1, as the name of the resistance gene in Hyuga. We also constructed a linkage map using recombinant inbred lines between Hyuga × Enrei using 176 SSR markers. We mapped Rpsv1 near the Satt435 locus on soybean chromosome 7. Japanese Society of Breeding 2012-01 2012-02-04 /pmc/articles/PMC3406795/ /pubmed/23136501 http://dx.doi.org/10.1270/jsbbs.61.625 Text en Copyright © 2012 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Saruta, Masayasu
Takada, Yoshitake
Kikuchi, Akio
Yamada, Tetsusya
Komatsu, Kunihiko
Sayama, Takashi
Ishimoto, Masao
Okabe, Akinori
Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene
title Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene
title_full Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene
title_fullStr Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene
title_full_unstemmed Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene
title_short Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene
title_sort screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative rpsv1 resistance gene
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406795/
https://www.ncbi.nlm.nih.gov/pubmed/23136501
http://dx.doi.org/10.1270/jsbbs.61.625
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