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Fluorine-19 Magnetic Resonance Angiography of the Mouse

PURPOSE: To implement and characterize a fluorine-19 ((19)F) magnetic resonance imaging (MRI) technique and to test the hypothesis that the (19)F MRI signal in steady state after intravenous injection of a perfluoro-15-crown-5 ether (PCE) emulsion may be exploited for angiography in a pre-clinical i...

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Autores principales: van Heeswijk, Ruud B., Pilloud, Yves, Flögel, Ulrich, Schwitter, Jürg, Stuber, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407132/
https://www.ncbi.nlm.nih.gov/pubmed/22848749
http://dx.doi.org/10.1371/journal.pone.0042236
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author van Heeswijk, Ruud B.
Pilloud, Yves
Flögel, Ulrich
Schwitter, Jürg
Stuber, Matthias
author_facet van Heeswijk, Ruud B.
Pilloud, Yves
Flögel, Ulrich
Schwitter, Jürg
Stuber, Matthias
author_sort van Heeswijk, Ruud B.
collection PubMed
description PURPOSE: To implement and characterize a fluorine-19 ((19)F) magnetic resonance imaging (MRI) technique and to test the hypothesis that the (19)F MRI signal in steady state after intravenous injection of a perfluoro-15-crown-5 ether (PCE) emulsion may be exploited for angiography in a pre-clinical in vivo animal study. MATERIALS AND METHODS: In vitro at 9.4T, the detection limit of the PCE emulsion at a scan time of 10 min/slice was determined, after which the T(1) and T(2) of PCE in venous blood were measured. Permission from the local animal use committee was obtained for all animal experiments. 12 µl/g of PCE emulsion was intravenously injected in 11 mice. Gradient echo (1)H and (19)F images were obtained at identical anatomical levels. Signal-to-noise (SNR) and contrast-to-noise (CNR) ratios were determined for 33 vessels in both the (19)F and (1)H images, which was followed by vessel tracking to determine the vessel conspicuity for both modalities. RESULTS: In vitro, the detection limit was ∼400 µM, while the (19)F T(1) and T(2) were 1350±40 and 25±2 ms. The (19)F MR angiograms selectively visualized the vasculature (and the liver parenchyma over time) while precisely coregistering with the (1)H images. Due to the lower SNR of (19)F compared to (1)H (17±8 vs. 83±49, p<0.001), the (19)F CNR was also lower at 15±8 vs. 52±35 (p<0.001). Vessel tracking demonstrated a significantly higher vessel sharpness in the (19)F images (66±11 vs. 56±12, p = 0.002). CONCLUSION: (19)F magnetic resonance angiography of intravenously administered perfluorocarbon emulsions is feasible for a selective and exclusive visualization of the vasculature in vivo.
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spelling pubmed-34071322012-07-30 Fluorine-19 Magnetic Resonance Angiography of the Mouse van Heeswijk, Ruud B. Pilloud, Yves Flögel, Ulrich Schwitter, Jürg Stuber, Matthias PLoS One Research Article PURPOSE: To implement and characterize a fluorine-19 ((19)F) magnetic resonance imaging (MRI) technique and to test the hypothesis that the (19)F MRI signal in steady state after intravenous injection of a perfluoro-15-crown-5 ether (PCE) emulsion may be exploited for angiography in a pre-clinical in vivo animal study. MATERIALS AND METHODS: In vitro at 9.4T, the detection limit of the PCE emulsion at a scan time of 10 min/slice was determined, after which the T(1) and T(2) of PCE in venous blood were measured. Permission from the local animal use committee was obtained for all animal experiments. 12 µl/g of PCE emulsion was intravenously injected in 11 mice. Gradient echo (1)H and (19)F images were obtained at identical anatomical levels. Signal-to-noise (SNR) and contrast-to-noise (CNR) ratios were determined for 33 vessels in both the (19)F and (1)H images, which was followed by vessel tracking to determine the vessel conspicuity for both modalities. RESULTS: In vitro, the detection limit was ∼400 µM, while the (19)F T(1) and T(2) were 1350±40 and 25±2 ms. The (19)F MR angiograms selectively visualized the vasculature (and the liver parenchyma over time) while precisely coregistering with the (1)H images. Due to the lower SNR of (19)F compared to (1)H (17±8 vs. 83±49, p<0.001), the (19)F CNR was also lower at 15±8 vs. 52±35 (p<0.001). Vessel tracking demonstrated a significantly higher vessel sharpness in the (19)F images (66±11 vs. 56±12, p = 0.002). CONCLUSION: (19)F magnetic resonance angiography of intravenously administered perfluorocarbon emulsions is feasible for a selective and exclusive visualization of the vasculature in vivo. Public Library of Science 2012-07-27 /pmc/articles/PMC3407132/ /pubmed/22848749 http://dx.doi.org/10.1371/journal.pone.0042236 Text en © 2012 van Heeswijk et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
van Heeswijk, Ruud B.
Pilloud, Yves
Flögel, Ulrich
Schwitter, Jürg
Stuber, Matthias
Fluorine-19 Magnetic Resonance Angiography of the Mouse
title Fluorine-19 Magnetic Resonance Angiography of the Mouse
title_full Fluorine-19 Magnetic Resonance Angiography of the Mouse
title_fullStr Fluorine-19 Magnetic Resonance Angiography of the Mouse
title_full_unstemmed Fluorine-19 Magnetic Resonance Angiography of the Mouse
title_short Fluorine-19 Magnetic Resonance Angiography of the Mouse
title_sort fluorine-19 magnetic resonance angiography of the mouse
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407132/
https://www.ncbi.nlm.nih.gov/pubmed/22848749
http://dx.doi.org/10.1371/journal.pone.0042236
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