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Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves
Despite Giardia duodenalis being one of the most commonly found intestinal pathogens in humans and animals, little is known about the host-parasite interactions in its natural hosts. Therefore, the objective of this study was to investigate the intestinal response in calves following a G. duodenalis...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407150/ https://www.ncbi.nlm.nih.gov/pubmed/22848418 http://dx.doi.org/10.1371/journal.pone.0040985 |
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author | Dreesen, Leentje Rinaldi, Manuela Chiers, Koen Li, Robert Geurden, Thomas Van den Broeck, Wim Goddeeris, Bruno Vercruysse, Jozef Claerebout, Edwin Geldhof, Peter |
author_facet | Dreesen, Leentje Rinaldi, Manuela Chiers, Koen Li, Robert Geurden, Thomas Van den Broeck, Wim Goddeeris, Bruno Vercruysse, Jozef Claerebout, Edwin Geldhof, Peter |
author_sort | Dreesen, Leentje |
collection | PubMed |
description | Despite Giardia duodenalis being one of the most commonly found intestinal pathogens in humans and animals, little is known about the host-parasite interactions in its natural hosts. Therefore, the objective of this study was to investigate the intestinal response in calves following a G. duodenalis infection, using a bovine high-density oligo microarray to analyze global gene expression in the small intestine. The resulting microarray data suggested a decrease in inflammation, immune response, and immune cell migration in infected animals. These findings were examined in more detail by histological analyses combined with quantitative real-time PCR on a panel of cytokines. The transcription levels of IL-6, IL-8, IL-13, IL-17, and IFN-γ showed a trend of being downregulated in the jejunum of infected animals compared to the negative controls,.No immune cell recruitment could be seen after infection, and no intestinal pathologies, such as villus shortening or increased levels of apoptosis. Possible regulators of this intestinal response are the nuclear peroxisome proliferator-activated receptors alpha (PPARα), and gamma (PPARγ) and the enzyme adenosine deaminase (ADA), all for which an upregulated expression was found in the microarray and qRT-PCR analyses. |
format | Online Article Text |
id | pubmed-3407150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34071502012-07-30 Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves Dreesen, Leentje Rinaldi, Manuela Chiers, Koen Li, Robert Geurden, Thomas Van den Broeck, Wim Goddeeris, Bruno Vercruysse, Jozef Claerebout, Edwin Geldhof, Peter PLoS One Research Article Despite Giardia duodenalis being one of the most commonly found intestinal pathogens in humans and animals, little is known about the host-parasite interactions in its natural hosts. Therefore, the objective of this study was to investigate the intestinal response in calves following a G. duodenalis infection, using a bovine high-density oligo microarray to analyze global gene expression in the small intestine. The resulting microarray data suggested a decrease in inflammation, immune response, and immune cell migration in infected animals. These findings were examined in more detail by histological analyses combined with quantitative real-time PCR on a panel of cytokines. The transcription levels of IL-6, IL-8, IL-13, IL-17, and IFN-γ showed a trend of being downregulated in the jejunum of infected animals compared to the negative controls,.No immune cell recruitment could be seen after infection, and no intestinal pathologies, such as villus shortening or increased levels of apoptosis. Possible regulators of this intestinal response are the nuclear peroxisome proliferator-activated receptors alpha (PPARα), and gamma (PPARγ) and the enzyme adenosine deaminase (ADA), all for which an upregulated expression was found in the microarray and qRT-PCR analyses. Public Library of Science 2012-07-27 /pmc/articles/PMC3407150/ /pubmed/22848418 http://dx.doi.org/10.1371/journal.pone.0040985 Text en © 2012 Dreesen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Dreesen, Leentje Rinaldi, Manuela Chiers, Koen Li, Robert Geurden, Thomas Van den Broeck, Wim Goddeeris, Bruno Vercruysse, Jozef Claerebout, Edwin Geldhof, Peter Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves |
title | Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves |
title_full | Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves |
title_fullStr | Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves |
title_full_unstemmed | Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves |
title_short | Microarray Analysis of the Intestinal Host Response in Giardia duodenalis Assemblage E Infected Calves |
title_sort | microarray analysis of the intestinal host response in giardia duodenalis assemblage e infected calves |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407150/ https://www.ncbi.nlm.nih.gov/pubmed/22848418 http://dx.doi.org/10.1371/journal.pone.0040985 |
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