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A Single-Step Purification and Molecular Characterization of Functional Shiga Toxin 2 Variants from Pathogenic Escherichia coli

A one-step affinity chromatography method was developed to purify Shiga toxin 2 variants (Stx2) Stx2a, Stx2c, Stx2d and Stx2g from bacterial culture supernatants. Analysis of the purified Stx2 variants by denaturing gel electrophoresis revealed 32 kDa and 7 kDa protein bands, corresponding to the St...

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Detalles Bibliográficos
Autores principales:	He, Xiaohua, Quiñones, Beatriz, McMahon, Stephanie, Mandrell, Robert E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407889/ https://www.ncbi.nlm.nih.gov/pubmed/22852065 http://dx.doi.org/10.3390/toxins4070487

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