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Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains

Interest in commercial cultivation and product development of Cordyceps species has shown a recent increase. Due to its biochemical and pharmacological effects, Cordyceps militaris, commonly known as orange caterpillar fungus, is being investigated with great interest. Cultivation of C. militaris ha...

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Autores principales: Shrestha, Bhushan, Han, Sang-Kuk, Sung, Jae-Mo, Sung, Gi-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408298/
https://www.ncbi.nlm.nih.gov/pubmed/22870051
http://dx.doi.org/10.5941/MYCO.2012.40.2.100
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author Shrestha, Bhushan
Han, Sang-Kuk
Sung, Jae-Mo
Sung, Gi-Ho
author_facet Shrestha, Bhushan
Han, Sang-Kuk
Sung, Jae-Mo
Sung, Gi-Ho
author_sort Shrestha, Bhushan
collection PubMed
description Interest in commercial cultivation and product development of Cordyceps species has shown a recent increase. Due to its biochemical and pharmacological effects, Cordyceps militaris, commonly known as orange caterpillar fungus, is being investigated with great interest. Cultivation of C. militaris has been practiced on a large scale in order to fulfill a demand for scientific investigation and product development. Isolates of C. militaris can be easily established from both spores and tissue. For isolation of spores, ascospores released from mature stromata are trapped in sterile medium. Multi-ascospore isolates, as well as combinations of single ascospore strains, are used for production of fruiting bodies. Progeny ascospore strains can be isolated from artificial fruiting bodies, thus, the cycle of fruiting body production can be continued for a long period of time. In this study, we examined fruiting body production from multi-ascospore isolates and their progeny strains for three generations. F(1) progeny strains generally produced a larger number of fruiting bodies, compared with their mother multi-ascospore isolates; however, F(2) and F(3) progeny strains produced fewer fruiting bodies. Optimum preservation conditions could help to increase the vitality of the progeny strains. In order to retain the fruiting ability of the strains, further testing of various methods of preservation and different methods for isolation should be performed.
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spelling pubmed-34082982012-08-06 Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains Shrestha, Bhushan Han, Sang-Kuk Sung, Jae-Mo Sung, Gi-Ho Mycobiology Research Article Interest in commercial cultivation and product development of Cordyceps species has shown a recent increase. Due to its biochemical and pharmacological effects, Cordyceps militaris, commonly known as orange caterpillar fungus, is being investigated with great interest. Cultivation of C. militaris has been practiced on a large scale in order to fulfill a demand for scientific investigation and product development. Isolates of C. militaris can be easily established from both spores and tissue. For isolation of spores, ascospores released from mature stromata are trapped in sterile medium. Multi-ascospore isolates, as well as combinations of single ascospore strains, are used for production of fruiting bodies. Progeny ascospore strains can be isolated from artificial fruiting bodies, thus, the cycle of fruiting body production can be continued for a long period of time. In this study, we examined fruiting body production from multi-ascospore isolates and their progeny strains for three generations. F(1) progeny strains generally produced a larger number of fruiting bodies, compared with their mother multi-ascospore isolates; however, F(2) and F(3) progeny strains produced fewer fruiting bodies. Optimum preservation conditions could help to increase the vitality of the progeny strains. In order to retain the fruiting ability of the strains, further testing of various methods of preservation and different methods for isolation should be performed. The Korean Society of Mycology 2012-06 2012-06-29 /pmc/articles/PMC3408298/ /pubmed/22870051 http://dx.doi.org/10.5941/MYCO.2012.40.2.100 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shrestha, Bhushan
Han, Sang-Kuk
Sung, Jae-Mo
Sung, Gi-Ho
Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains
title Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains
title_full Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains
title_fullStr Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains
title_full_unstemmed Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains
title_short Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains
title_sort fruiting body formation of cordyceps militaris from multi-ascospore isolates and their single ascospore progeny strains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408298/
https://www.ncbi.nlm.nih.gov/pubmed/22870051
http://dx.doi.org/10.5941/MYCO.2012.40.2.100
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