Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide...

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Autores principales: Chukeatirote, Ekachai, Maharachchikumbura, Sajeewa S. N., Wongkham, Shannaphimon, Sysouphanthong, Phongeun, Phookamsak, Rungtiwa, Hyde, Kevin D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408299/
https://www.ncbi.nlm.nih.gov/pubmed/22870052
http://dx.doi.org/10.5941/MYCO.2012.40.2.107
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author Chukeatirote, Ekachai
Maharachchikumbura, Sajeewa S. N.
Wongkham, Shannaphimon
Sysouphanthong, Phongeun
Phookamsak, Rungtiwa
Hyde, Kevin D.
author_facet Chukeatirote, Ekachai
Maharachchikumbura, Sajeewa S. N.
Wongkham, Shannaphimon
Sysouphanthong, Phongeun
Phookamsak, Rungtiwa
Hyde, Kevin D.
author_sort Chukeatirote, Ekachai
collection PubMed
description Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.
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spelling pubmed-34082992012-08-06 Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes Chukeatirote, Ekachai Maharachchikumbura, Sajeewa S. N. Wongkham, Shannaphimon Sysouphanthong, Phongeun Phookamsak, Rungtiwa Hyde, Kevin D. Mycobiology Research Article Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7. The Korean Society of Mycology 2012-06 2012-06-29 /pmc/articles/PMC3408299/ /pubmed/22870052 http://dx.doi.org/10.5941/MYCO.2012.40.2.107 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chukeatirote, Ekachai
Maharachchikumbura, Sajeewa S. N.
Wongkham, Shannaphimon
Sysouphanthong, Phongeun
Phookamsak, Rungtiwa
Hyde, Kevin D.
Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes
title Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes
title_full Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes
title_fullStr Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes
title_full_unstemmed Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes
title_short Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes
title_sort cloning and sequence analysis of the cellobiohydrolase i genes from some basidiomycetes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408299/
https://www.ncbi.nlm.nih.gov/pubmed/22870052
http://dx.doi.org/10.5941/MYCO.2012.40.2.107
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