Neuraminidase Inhibitors from the Culture Broth of Phellinus linteus

During the search for neuraminidase inhibitors from medicinal fungi, we found that the culture broth of Phellinus linteus exhibited potent inhibitory activity. Solvent partition, Sephadex LH-20 column chromatography, and high-performance liquid chromatography (HPLC) were performed for purification o...

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Detalles Bibliográficos
Autores principales: Yeom, Ji-Hee, Lee, In-Kyoung, Ki, Dae-Won, Lee, Myeong-Seok, Seok, Soon-Ja, Yun, Bong-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2012
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408306/
https://www.ncbi.nlm.nih.gov/pubmed/22870059
http://dx.doi.org/10.5941/MYCO.2012.40.2.142
Descripción
Sumario:During the search for neuraminidase inhibitors from medicinal fungi, we found that the culture broth of Phellinus linteus exhibited potent inhibitory activity. Solvent partition, Sephadex LH-20 column chromatography, and high-performance liquid chromatography (HPLC) were performed for purification of two active substances from the culture broth. According to (1)H NMR measurements and comparison of HPLC retention times with those of authentic compounds, their chemical structures were identified as hispidin and hypholomine B. Compounds (hispidin) 1 and 2 (hypholomine B) inhibited neuraminidase, with IC(50) values of 13.1 and 0.03 µM, respectively.

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