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The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation
Leukocyte transendothelial migration involves the active participation of the endothelium through the formation of apical membrane protrusions that embrace adherent leukocytes, termed docking structures. Using live-cell imaging, we find that prior to transmigration, endothelial docking structures fo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408411/ https://www.ncbi.nlm.nih.gov/pubmed/22696684 http://dx.doi.org/10.1091/mbc.E11-11-0907 |
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author | van Rijssel, Jos Kroon, Jeffrey Hoogenboezem, Mark van Alphen, Floris P. J. de Jong, Renske J. Kostadinova, Elena Geerts, Dirk Hordijk, Peter L. van Buul, Jaap D. |
author_facet | van Rijssel, Jos Kroon, Jeffrey Hoogenboezem, Mark van Alphen, Floris P. J. de Jong, Renske J. Kostadinova, Elena Geerts, Dirk Hordijk, Peter L. van Buul, Jaap D. |
author_sort | van Rijssel, Jos |
collection | PubMed |
description | Leukocyte transendothelial migration involves the active participation of the endothelium through the formation of apical membrane protrusions that embrace adherent leukocytes, termed docking structures. Using live-cell imaging, we find that prior to transmigration, endothelial docking structures form around 80% of all neutrophils. Previously we showed that endothelial RhoG and SGEF control leukocyte transmigration. In this study, our data reveal that both full-length Trio and the first DH-PH (TrioD1) domain of Trio, which can activate Rac1 and RhoG, interact with ICAM-1 and are recruited to leukocyte adhesion sites. Moreover, upon clustering of ICAM-1, the Rho-guanine nucleotide exchange factor Trio activates Rac1, prior to activating RhoG, in a filamin-dependent manner. We further show that docking structure formation is initiated by ICAM-1 clustering into ring-like structures, which is followed by apical membrane protrusion. Interestingly, we find that Rac1 is required for ICAM-1 clustering, whereas RhoG controls membrane protrusion formation. Finally, silencing endothelial Trio expression or reducing TrioD1 activity without affecting SGEF impairs both docking structure formation and leukocyte transmigration. We conclude that Trio promotes leukocyte transendothelial migration by inducing endothelial docking structure formation in a filamin-dependent manner through the activation of Rac1 and RhoG. |
format | Online Article Text |
id | pubmed-3408411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-34084112012-10-16 The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation van Rijssel, Jos Kroon, Jeffrey Hoogenboezem, Mark van Alphen, Floris P. J. de Jong, Renske J. Kostadinova, Elena Geerts, Dirk Hordijk, Peter L. van Buul, Jaap D. Mol Biol Cell Articles Leukocyte transendothelial migration involves the active participation of the endothelium through the formation of apical membrane protrusions that embrace adherent leukocytes, termed docking structures. Using live-cell imaging, we find that prior to transmigration, endothelial docking structures form around 80% of all neutrophils. Previously we showed that endothelial RhoG and SGEF control leukocyte transmigration. In this study, our data reveal that both full-length Trio and the first DH-PH (TrioD1) domain of Trio, which can activate Rac1 and RhoG, interact with ICAM-1 and are recruited to leukocyte adhesion sites. Moreover, upon clustering of ICAM-1, the Rho-guanine nucleotide exchange factor Trio activates Rac1, prior to activating RhoG, in a filamin-dependent manner. We further show that docking structure formation is initiated by ICAM-1 clustering into ring-like structures, which is followed by apical membrane protrusion. Interestingly, we find that Rac1 is required for ICAM-1 clustering, whereas RhoG controls membrane protrusion formation. Finally, silencing endothelial Trio expression or reducing TrioD1 activity without affecting SGEF impairs both docking structure formation and leukocyte transmigration. We conclude that Trio promotes leukocyte transendothelial migration by inducing endothelial docking structure formation in a filamin-dependent manner through the activation of Rac1 and RhoG. The American Society for Cell Biology 2012-08-01 /pmc/articles/PMC3408411/ /pubmed/22696684 http://dx.doi.org/10.1091/mbc.E11-11-0907 Text en © 2012 van Rijssel et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles van Rijssel, Jos Kroon, Jeffrey Hoogenboezem, Mark van Alphen, Floris P. J. de Jong, Renske J. Kostadinova, Elena Geerts, Dirk Hordijk, Peter L. van Buul, Jaap D. The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation |
title | The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation |
title_full | The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation |
title_fullStr | The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation |
title_full_unstemmed | The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation |
title_short | The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation |
title_sort | rho-guanine nucleotide exchange factor trio controls leukocyte transendothelial migration by promoting docking structure formation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408411/ https://www.ncbi.nlm.nih.gov/pubmed/22696684 http://dx.doi.org/10.1091/mbc.E11-11-0907 |
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