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Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a

The generation of myelinating cells from multipotential neural stem cells in the CNS requires the initiation of specific gene expression programs in oligodendrocytes (OLs). We reasoned that microRNAs (miRNAs) could play an important role in this process by regulating genes crucial for OL development...

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Detalles Bibliográficos
Autores principales: Zhao, Xianghui, Wu, Jiang, Zheng, Minhua, Gao, Fang, Ju, Gong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408414/
https://www.ncbi.nlm.nih.gov/pubmed/22696677
http://dx.doi.org/10.1091/mbc.E12-04-0270
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author Zhao, Xianghui
Wu, Jiang
Zheng, Minhua
Gao, Fang
Ju, Gong
author_facet Zhao, Xianghui
Wu, Jiang
Zheng, Minhua
Gao, Fang
Ju, Gong
author_sort Zhao, Xianghui
collection PubMed
description The generation of myelinating cells from multipotential neural stem cells in the CNS requires the initiation of specific gene expression programs in oligodendrocytes (OLs). We reasoned that microRNAs (miRNAs) could play an important role in this process by regulating genes crucial for OL development. Here we identified miR-7a as one of the highly enriched miRNAs in oligodendrocyte precursor cells (OPCs), overexpression of which in either neural progenitor cells (NPCs) or embryonic mouse cortex promoted the generation of OL lineage cells. Blocking the function of miR-7a in differentiating NPCs led to a reduction in OL number and an expansion of neuronal populations simultaneously. We also found that overexpression of this miRNA in purified OPC cultures promoted cell proliferation and inhibited further maturation. In addition, miR-7a might exert the effects just mentioned partially by directly repressing proneuronal differentiation factors including Pax6 and NeuroD4, or proOL genes involved in oligodendrocyte maturation. These results suggest that miRNA pathway is essential in determining cell fate commitment for OLs and thus providing a new strategy for modulating this process in OL loss diseases.
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spelling pubmed-34084142012-10-16 Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a Zhao, Xianghui Wu, Jiang Zheng, Minhua Gao, Fang Ju, Gong Mol Biol Cell Articles The generation of myelinating cells from multipotential neural stem cells in the CNS requires the initiation of specific gene expression programs in oligodendrocytes (OLs). We reasoned that microRNAs (miRNAs) could play an important role in this process by regulating genes crucial for OL development. Here we identified miR-7a as one of the highly enriched miRNAs in oligodendrocyte precursor cells (OPCs), overexpression of which in either neural progenitor cells (NPCs) or embryonic mouse cortex promoted the generation of OL lineage cells. Blocking the function of miR-7a in differentiating NPCs led to a reduction in OL number and an expansion of neuronal populations simultaneously. We also found that overexpression of this miRNA in purified OPC cultures promoted cell proliferation and inhibited further maturation. In addition, miR-7a might exert the effects just mentioned partially by directly repressing proneuronal differentiation factors including Pax6 and NeuroD4, or proOL genes involved in oligodendrocyte maturation. These results suggest that miRNA pathway is essential in determining cell fate commitment for OLs and thus providing a new strategy for modulating this process in OL loss diseases. The American Society for Cell Biology 2012-08-01 /pmc/articles/PMC3408414/ /pubmed/22696677 http://dx.doi.org/10.1091/mbc.E12-04-0270 Text en © 2012 Zhao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Zhao, Xianghui
Wu, Jiang
Zheng, Minhua
Gao, Fang
Ju, Gong
Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a
title Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a
title_full Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a
title_fullStr Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a
title_full_unstemmed Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a
title_short Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a
title_sort specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microrna-7a
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408414/
https://www.ncbi.nlm.nih.gov/pubmed/22696677
http://dx.doi.org/10.1091/mbc.E12-04-0270
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