Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues

A photon-tissue interaction (PTI) model was developed and employed to analyze 96 pairs of reflectance and fluorescence spectra from freshly excised human pancreatic tissues. For each pair of spectra, the PTI model extracted a cellular nuclear size parameter from the measured reflectance, and the rel...

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Detalles Bibliográficos
Autores principales: Wilson, Robert H., Chandra, Malavika, Chen, Leng-Chun, Lloyd, William R., Scheiman, James, Simeone, Diane, Purdy, Julianne, McKenna, Barbara, Mycek, Mary-Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Research-Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408914/
https://www.ncbi.nlm.nih.gov/pubmed/20941059
http://dx.doi.org/10.1364/OE.18.021612
Descripción
Sumario:A photon-tissue interaction (PTI) model was developed and employed to analyze 96 pairs of reflectance and fluorescence spectra from freshly excised human pancreatic tissues. For each pair of spectra, the PTI model extracted a cellular nuclear size parameter from the measured reflectance, and the relative contributions of extracellular and intracellular fluorophores to the intrinsic fluorescence. The results suggest that reflectance and fluorescence spectroscopies have the potential to quantitatively distinguish among pancreatic tissue types, including normal pancreatic tissue, pancreatitis, and pancreatic adenocarcinoma.

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