Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues

A photon-tissue interaction (PTI) model was developed and employed to analyze 96 pairs of reflectance and fluorescence spectra from freshly excised human pancreatic tissues. For each pair of spectra, the PTI model extracted a cellular nuclear size parameter from the measured reflectance, and the rel...

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Autores principales: Wilson, Robert H., Chandra, Malavika, Chen, Leng-Chun, Lloyd, William R., Scheiman, James, Simeone, Diane, Purdy, Julianne, McKenna, Barbara, Mycek, Mary-Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Research-Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408914/
https://www.ncbi.nlm.nih.gov/pubmed/20941059
http://dx.doi.org/10.1364/OE.18.021612
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author Wilson, Robert H.
Chandra, Malavika
Chen, Leng-Chun
Lloyd, William R.
Scheiman, James
Simeone, Diane
Purdy, Julianne
McKenna, Barbara
Mycek, Mary-Ann
author_facet Wilson, Robert H.
Chandra, Malavika
Chen, Leng-Chun
Lloyd, William R.
Scheiman, James
Simeone, Diane
Purdy, Julianne
McKenna, Barbara
Mycek, Mary-Ann
author_sort Wilson, Robert H.
collection PubMed
description A photon-tissue interaction (PTI) model was developed and employed to analyze 96 pairs of reflectance and fluorescence spectra from freshly excised human pancreatic tissues. For each pair of spectra, the PTI model extracted a cellular nuclear size parameter from the measured reflectance, and the relative contributions of extracellular and intracellular fluorophores to the intrinsic fluorescence. The results suggest that reflectance and fluorescence spectroscopies have the potential to quantitatively distinguish among pancreatic tissue types, including normal pancreatic tissue, pancreatitis, and pancreatic adenocarcinoma.
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spelling pubmed-34089142012-10-01 Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues Wilson, Robert H. Chandra, Malavika Chen, Leng-Chun Lloyd, William R. Scheiman, James Simeone, Diane Purdy, Julianne McKenna, Barbara Mycek, Mary-Ann Opt Express Research-Article A photon-tissue interaction (PTI) model was developed and employed to analyze 96 pairs of reflectance and fluorescence spectra from freshly excised human pancreatic tissues. For each pair of spectra, the PTI model extracted a cellular nuclear size parameter from the measured reflectance, and the relative contributions of extracellular and intracellular fluorophores to the intrinsic fluorescence. The results suggest that reflectance and fluorescence spectroscopies have the potential to quantitatively distinguish among pancreatic tissue types, including normal pancreatic tissue, pancreatitis, and pancreatic adenocarcinoma. Optical Society of America 2010-09-28 /pmc/articles/PMC3408914/ /pubmed/20941059 http://dx.doi.org/10.1364/OE.18.021612 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Wilson, Robert H.
Chandra, Malavika
Chen, Leng-Chun
Lloyd, William R.
Scheiman, James
Simeone, Diane
Purdy, Julianne
McKenna, Barbara
Mycek, Mary-Ann
Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues
title Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues
title_full Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues
title_fullStr Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues
title_full_unstemmed Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues
title_short Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues
title_sort photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408914/
https://www.ncbi.nlm.nih.gov/pubmed/20941059
http://dx.doi.org/10.1364/OE.18.021612
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