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Removal of subsurface fluorescence in cryo-imaging using deconvolution

We compared image restoration methods [Richardson-Lucy (RL), Wiener, and Next-image] with measured “scatter” point-spread-functions, for removing subsurface fluorescence from section-and-image cryo-image volumes. All methods removed haze, delineated single cells from clusters, and improved visualiza...

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Detalles Bibliográficos
Autores principales: Krishnamurthi, Ganapathy, Wang, Charlie Y., Steyer, Grant, Wilson, David L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408948/
https://www.ncbi.nlm.nih.gov/pubmed/20941133
http://dx.doi.org/10.1364/OE.18.022324

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