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Removal of subsurface fluorescence in cryo-imaging using deconvolution
We compared image restoration methods [Richardson-Lucy (RL), Wiener, and Next-image] with measured “scatter” point-spread-functions, for removing subsurface fluorescence from section-and-image cryo-image volumes. All methods removed haze, delineated single cells from clusters, and improved visualiza...
Autores principales: | Krishnamurthi, Ganapathy, Wang, Charlie Y., Steyer, Grant, Wilson, David L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408948/ https://www.ncbi.nlm.nih.gov/pubmed/20941133 http://dx.doi.org/10.1364/OE.18.022324 |
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