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Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1

Bacterial biofilm formation starts with single cells attaching to a surface, however, little is known about the initial attachment steps and the adaptation to the surface-associated life style. Here, we describe a hydrodynamic system that allows easy harvest of cells at very early biofilm stages. Us...

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Detalles Bibliográficos
Autores principales: Gödeke, Julia, Binnenkade, Lucas, Thormann, Kai M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409153/
https://www.ncbi.nlm.nih.gov/pubmed/22860070
http://dx.doi.org/10.1371/journal.pone.0042160
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author Gödeke, Julia
Binnenkade, Lucas
Thormann, Kai M.
author_facet Gödeke, Julia
Binnenkade, Lucas
Thormann, Kai M.
author_sort Gödeke, Julia
collection PubMed
description Bacterial biofilm formation starts with single cells attaching to a surface, however, little is known about the initial attachment steps and the adaptation to the surface-associated life style. Here, we describe a hydrodynamic system that allows easy harvest of cells at very early biofilm stages. Using the metal ion-reducing gammaproteobacterium Shewanella oneidensis MR-1 as a model organism, we analyzed the transcriptional changes occurring during surface-associated growth between 15 and 60 minutes after attachment. 230 genes were significantly upregulated and 333 were downregulated by a factor of ≥2. Main functional categories of the corresponding gene products comprise metabolism, uptake and transport, regulation, and hypothetical proteins. Among the genes highly upregulated those implicated in iron uptake are highly overrepresented, strongly indicating that S. oneidensis MR-1 has a high demand for iron during surface attachment and initial biofilm stages. Subsequent microscopic analysis of biofilm formation under hydrodynamic conditions revealed that addition of Fe(II) significantly stimulated biofilm formation of S. oneidensis MR-1 while planktonic growth was not affected. Our approach to harvest cells for transcriptional analysis of early biofilm stages is expected to be easily adapted to other bacterial species.
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spelling pubmed-34091532012-08-02 Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1 Gödeke, Julia Binnenkade, Lucas Thormann, Kai M. PLoS One Research Article Bacterial biofilm formation starts with single cells attaching to a surface, however, little is known about the initial attachment steps and the adaptation to the surface-associated life style. Here, we describe a hydrodynamic system that allows easy harvest of cells at very early biofilm stages. Using the metal ion-reducing gammaproteobacterium Shewanella oneidensis MR-1 as a model organism, we analyzed the transcriptional changes occurring during surface-associated growth between 15 and 60 minutes after attachment. 230 genes were significantly upregulated and 333 were downregulated by a factor of ≥2. Main functional categories of the corresponding gene products comprise metabolism, uptake and transport, regulation, and hypothetical proteins. Among the genes highly upregulated those implicated in iron uptake are highly overrepresented, strongly indicating that S. oneidensis MR-1 has a high demand for iron during surface attachment and initial biofilm stages. Subsequent microscopic analysis of biofilm formation under hydrodynamic conditions revealed that addition of Fe(II) significantly stimulated biofilm formation of S. oneidensis MR-1 while planktonic growth was not affected. Our approach to harvest cells for transcriptional analysis of early biofilm stages is expected to be easily adapted to other bacterial species. Public Library of Science 2012-07-31 /pmc/articles/PMC3409153/ /pubmed/22860070 http://dx.doi.org/10.1371/journal.pone.0042160 Text en © 2012 Gödeke et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gödeke, Julia
Binnenkade, Lucas
Thormann, Kai M.
Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1
title Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1
title_full Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1
title_fullStr Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1
title_full_unstemmed Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1
title_short Transcriptome Analysis of Early Surface-Associated Growth of Shewanella oneidensis MR-1
title_sort transcriptome analysis of early surface-associated growth of shewanella oneidensis mr-1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409153/
https://www.ncbi.nlm.nih.gov/pubmed/22860070
http://dx.doi.org/10.1371/journal.pone.0042160
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