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Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates

The evolution of the H5N1 highly pathogenic avian influenza virus (HPAIV) has resulted in high sequence variations and diverse antigenic properties in circulating viral isolates. We investigated immune responses induced by HA DNA vaccines of two contemporary H5N1 HPAIV isolates, A/bar-headed goose/Q...

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Autores principales: Gao, Yulong, Wen, Zhiyuan, Dong, Ke, Zhong, Gongxun, Wang, Xiaomei, Bu, Zhigao, Chen, Hualan, Ye, Ling, Yang, Chinglai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409192/
https://www.ncbi.nlm.nih.gov/pubmed/22859976
http://dx.doi.org/10.1371/journal.pone.0041332
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author Gao, Yulong
Wen, Zhiyuan
Dong, Ke
Zhong, Gongxun
Wang, Xiaomei
Bu, Zhigao
Chen, Hualan
Ye, Ling
Yang, Chinglai
author_facet Gao, Yulong
Wen, Zhiyuan
Dong, Ke
Zhong, Gongxun
Wang, Xiaomei
Bu, Zhigao
Chen, Hualan
Ye, Ling
Yang, Chinglai
author_sort Gao, Yulong
collection PubMed
description The evolution of the H5N1 highly pathogenic avian influenza virus (HPAIV) has resulted in high sequence variations and diverse antigenic properties in circulating viral isolates. We investigated immune responses induced by HA DNA vaccines of two contemporary H5N1 HPAIV isolates, A/bar-headed goose/Qinghai/3/2005 (QH) and A/chicken/Shanxi/2/2006 (SX) respectively, against the homologous as well as the heterologous virus isolate for comparison. Characterization of antibody responses induced by immunization with QH-HA and SX-HA DNA vaccines showed that the two isolates are antigenically distinctive. Interestingly, after immunization with the QH-HA DNA vaccine, subsequent boosting with the SX-HA DNA vaccine significantly augmented antibody responses against the QH isolate but only induced low levels of antibody responses against the SX isolate. Conversely, after immunization with the SX-HA DNA vaccine, subsequent boosting with the QH-HA DNA vaccine significantly augmented antibody responses against the SX isolate but only induced low levels of antibody responses against the QH isolate. In contrast to the antibody responses, cross-reactive T cell responses are readily detected between these two isolates at similar levels. These results indicate the existence of original antigenic sin (OAS) between concurrently circulating H5N1 HPAIV strains, which may need to be taken into consideration in vaccine development against the potential H5N1 HPAIV pandemic.
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spelling pubmed-34091922012-08-02 Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates Gao, Yulong Wen, Zhiyuan Dong, Ke Zhong, Gongxun Wang, Xiaomei Bu, Zhigao Chen, Hualan Ye, Ling Yang, Chinglai PLoS One Research Article The evolution of the H5N1 highly pathogenic avian influenza virus (HPAIV) has resulted in high sequence variations and diverse antigenic properties in circulating viral isolates. We investigated immune responses induced by HA DNA vaccines of two contemporary H5N1 HPAIV isolates, A/bar-headed goose/Qinghai/3/2005 (QH) and A/chicken/Shanxi/2/2006 (SX) respectively, against the homologous as well as the heterologous virus isolate for comparison. Characterization of antibody responses induced by immunization with QH-HA and SX-HA DNA vaccines showed that the two isolates are antigenically distinctive. Interestingly, after immunization with the QH-HA DNA vaccine, subsequent boosting with the SX-HA DNA vaccine significantly augmented antibody responses against the QH isolate but only induced low levels of antibody responses against the SX isolate. Conversely, after immunization with the SX-HA DNA vaccine, subsequent boosting with the QH-HA DNA vaccine significantly augmented antibody responses against the SX isolate but only induced low levels of antibody responses against the QH isolate. In contrast to the antibody responses, cross-reactive T cell responses are readily detected between these two isolates at similar levels. These results indicate the existence of original antigenic sin (OAS) between concurrently circulating H5N1 HPAIV strains, which may need to be taken into consideration in vaccine development against the potential H5N1 HPAIV pandemic. Public Library of Science 2012-07-31 /pmc/articles/PMC3409192/ /pubmed/22859976 http://dx.doi.org/10.1371/journal.pone.0041332 Text en © 2012 Gao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gao, Yulong
Wen, Zhiyuan
Dong, Ke
Zhong, Gongxun
Wang, Xiaomei
Bu, Zhigao
Chen, Hualan
Ye, Ling
Yang, Chinglai
Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
title Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
title_full Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
title_fullStr Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
title_full_unstemmed Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
title_short Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
title_sort characterization of immune responses induced by immunization with the ha dna vaccines of two antigenically distinctive h5n1 hpaiv isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409192/
https://www.ncbi.nlm.nih.gov/pubmed/22859976
http://dx.doi.org/10.1371/journal.pone.0041332
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