Validation of the β-amy1 Transcription Profiling Assay and Selection of Reference Genes Suited for a RT-qPCR Assay in Developing Barley Caryopsis

Reverse transcription coupled with real-time quantitative PCR (RT-qPCR) is a frequently used method for gene expression profiling. Reference genes (RGs) are commonly employed to normalize gene expression data. A limited information exist on the gene expression and profiling in developing barley cary...

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Detalles Bibliográficos
Autores principales: Ovesná, Jaroslava, Kučera, Ladislav, Vaculová, Kateřina, Štrymplová, Kamila, Svobodová, Ilona, Milella, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409215/
https://www.ncbi.nlm.nih.gov/pubmed/22860024
http://dx.doi.org/10.1371/journal.pone.0041886

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